National and Local Joint Engineering Research Center of Technical Fiber Composites for Safety and Health, School of Textile and Clothing, Nantong University, Nantong 226019, China.
National and Local Joint Engineering Research Center of Technical Fiber Composites for Safety and Health, School of Textile and Clothing, Nantong University, Nantong 226019, China.
J Colloid Interface Sci. 2021 Dec;603:771-782. doi: 10.1016/j.jcis.2021.06.155. Epub 2021 Jun 30.
In this work, a metal-organic framework material, zeolitic imidazolate framework-90 (ZIF-90), was firstly used to encapsulate laccase (LAC) and to prepare ZIF-90/LAC biocomposites. Afterward, the composites were combined with bacterial cellulose (BC) and carboxylated multi-walled carbon nanotubes (c-MWCNTs) by a facile method to achieve a novel cellulose membrane with biocatalytic function, displaying excellent detection and degradation properties towards phenolic pollutant. Notably, the membrane was directly employed as a biosensor electrode, and it exhibited a linear response to catechol from 20 to 400 μM with a detection limit of 1.86 µM (S/N = 3), as well as satisfactory selectivity, reproducibility, and stability. In addition, the biocatalytic membrane showed higher degradation efficiency towards catechol than pure LAC, and the catechol degradation efficiency of the membrane generally ranged from 93.4% to 82.1% for five cycles. Moreover, the membrane was successfully applied in enzyme membrane reactor (EMR), achieving satisfactory results. The novel membrane harbors a broad application prospect in the fields of real-time monitor and treatment of phenolic wastewater.
在这项工作中，首次使用金属有机骨架材料沸石咪唑酯骨架-90（ZIF-90）包封漆酶（LAC），制备 ZIF-90/LAC 生物复合材料。随后，通过简便的方法将复合材料与细菌纤维素（BC）和羧基化多壁碳纳米管（c-MWCNTs）结合，得到具有生物催化功能的新型纤维素膜，对酚类污染物表现出优异的检测和降解性能。值得注意的是，该膜可直接用作生物传感器电极，对儿茶酚的线性响应范围为 20 至 400 μM，检测限为 1.86 μM（S/N=3），具有良好的选择性、重现性和稳定性。此外，与纯 LAC 相比，生物催化膜对儿茶酚的降解效率更高，在 5 个循环中，该膜对儿茶酚的降解效率一般在 93.4%至 82.1%之间。此外，该膜已成功应用于酶膜反应器（EMR）中，取得了满意的效果。这种新型膜在实时监测和处理酚废水领域具有广阔的应用前景。
