Schraermeyer U, Peters W, Zahner H
Institut für Zoologie II, Universität Düsseldorf, Federal Republic of Germany.
Parasitol Res. 1987;73(6):550-6. doi: 10.1007/BF00535332.
Sections of macrofilariae of Brugia malayi and Litomosoides carinii revealed binding of the gold-labelled lectins WGA, DBA and PNA. Specificity of binding was controlled by competitive inhibition with the respective sugars. N-acetyl-glucosamine, N-acetylgalactosamine and galactose residues seem to be present in the respective tissues. The lectins were bound preferentially to parts of the reproductive organs and to the fluid contents of their lumina. The results of the chitosan test and binding experiments with WGA-gold conjugate suggest the presence of chitin in the sheath of oocytes or zygotes. Binding of WGA could not be inhibited with 0.5 M N-acetylglucosamine, but only with 10 mM triacetyl chitotriose. In older stages, binding of WGA to the sheath could be inhibited by 0.5 M N-acetylglucosamine. In mature microfilariae, the outer surface of the sheath did not show affinity for WGA, but small amounts were bound to the inner surface. Therefore, the sheath of later developmental stages and microfilariae does not contain chitin but only N-acetylglucosamine residues. The degradation of the chitin content might enable the elongation and flexibility of the sheath of microfilariae.
马来布鲁线虫和卡里尼丝虫的成虫切片显示，金标记的凝集素WGA、DBA和PNA存在结合现象。结合的特异性通过与相应糖类的竞争性抑制来控制。N-乙酰葡糖胺、N-乙酰半乳糖胺和半乳糖残基似乎存在于各自的组织中。凝集素优先结合于生殖器官的部分以及其管腔的液体内容物。壳聚糖试验以及用WGA-金偶联物进行的结合实验结果表明，卵母细胞或受精卵的鞘中存在几丁质。0.5M N-乙酰葡糖胺不能抑制WGA的结合，只有10mM三乙酰壳三糖才能抑制。在较老阶段，0.5M N-乙酰葡糖胺可抑制WGA与鞘的结合。在成熟微丝蚴中，鞘的外表面对WGA没有亲和力，但有少量结合于内表面。因此，后期发育阶段和微丝蚴的鞘不含几丁质，只含N-乙酰葡糖胺残基。几丁质含量的降解可能使微丝蚴鞘能够伸长并具有柔韧性。
