Al Musawi Safiya, Ur Rahman Jawad, Aljaroodi Salma Ali, AlShammari Lateefah, Itbaileh Ahmed, Mohammed Hessa, Saeed Nermin, Abdalhamid Baha, Alkharsah Khaled R, Aljindan Reem Y
Department of Microbiology, College of Medicine, Imam Abdulrahman Bin Faisal University (IAU), Dammam, Saudi Arabia.
Department of Pathology and Laboratory Medicine, King Fahad Specialist Hospital, Dammam, Saudi Arabia.
J Med Microbiol. 2021 Jul;70(7). doi: 10.1099/jmm.0.001381.
Carbapenem resistant (CRE) are one of the leading causes of systemic and nosocomial infections and are multidrug-resistant organisms producing different carbapenemases. There are many genotypic and phenotypic methods for detecting the carbapenemases; however, there is a limitation for each. Modified carbapenem inactivation method (mCIM) assay is a recent phenotypic method which has been published by the Clinical and Laboratory Standards Institute. mCIM assay could provide a reliable method for the detection of carbapenemases in CRE. Evaluation of the mCIM assay performance for the detection of carbapenemases in and the identification of the common carbapenemase genes at Eastern Province of Saudi Arabia and Kingdom of Bahrain. A collection of 197 non-duplicate carbapenem resistant clinical isolates, were evaluated with the mCIM test comparing its performance to multiplex PCR. The minimum inhibitory concentration susceptibility testing was done by the Etest method for imipenem, meropenem, and ertapenem. The sensitivity of the mCIM assay was 94 % (95 % CI, (89.3-97.1)). In Saudi Arabia and Bahrain, OXA-48 was the most prevalent carbapenemase gene followed by NDM. Coexistence of multiple carbapenemase genes is reported in eleven cases. These findings indicate that the mCIM test is a reliable and simple assay for detecting the activity of carbapenemase in , especially in resource-limited laboratories.
耐碳青霉烯类肠杆菌科细菌（CRE）是引起全身性感染和医院感染的主要原因之一，是产生不同碳青霉烯酶的多重耐药菌。有许多检测碳青霉烯酶的基因型和表型方法；然而，每种方法都有局限性。改良碳青霉烯灭活法（mCIM）检测是临床和实验室标准协会最近公布的一种表型方法。mCIM检测可为检测CRE中的碳青霉烯酶提供一种可靠的方法。评估mCIM检测在沙特阿拉伯东部省和巴林王国检测碳青霉烯酶的性能以及鉴定常见碳青霉烯酶基因。收集了197株非重复的耐碳青霉烯类临床分离株，用mCIM试验进行评估，并将其性能与多重PCR进行比较。采用Etest法对亚胺培南、美罗培南和厄他培南进行最低抑菌浓度药敏试验。mCIM检测的敏感性为94%（95%CI，（89.3-97.1））。在沙特阿拉伯和巴林，OXA-48是最常见的碳青霉烯酶基因，其次是NDM。11例报告了多种碳青霉烯酶基因共存的情况。这些发现表明，mCIM检测是一种可靠且简单的检测CRE中碳青霉烯酶活性的方法，尤其是在资源有限的实验室。
