拮抗 PAR 蛋白之间的平衡决定了 C. elegans 胚胎发生中不对称和对称分裂的模式。
A balance between antagonizing PAR proteins specifies the pattern of asymmetric and symmetric divisions in C. elegans embryogenesis.
机构信息
Temasek Life-sciences Laboratory, Singapore 117604, Singapore; Department of Biological Sciences, National University of Singapore, Singapore 117583, Singapore.
RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan.
出版信息
Cell Rep. 2021 Jul 6;36(1):109326. doi: 10.1016/j.celrep.2021.109326.
Coordination between cell differentiation and proliferation during development requires the balance between asymmetric and symmetric modes of cell division. However, the cellular intrinsic cue underlying the choice between these two division modes remains elusive. Here, we show evidence in Caenorhabditis elegans that the invariable lineage of the division modes is specified by the balance between antagonizing complexes of partitioning-defective (PAR) proteins. By uncoupling unequal inheritance of PAR proteins from that of fate determinants during cell division, we demonstrate that changes in the balance between PAR-2 and PAR-6 can be sufficient to re-program the division modes from symmetric to asymmetric and vice versa in two daughter cells. The division mode adopted occurs independently of asymmetry in cytoplasmic fate determinants, cell-size asymmetry, and cell-cycle asynchrony between sister cells. We propose that the balance between PAR proteins represents an intrinsic self-organizing cue for the specification of the two division modes during development.
在发育过程中，细胞分化和增殖之间的协调需要在不对称和对称的细胞分裂模式之间取得平衡。然而，这两种分裂模式之间选择的细胞内在线索仍然难以捉摸。在这里，我们在秀丽隐杆线虫中表明，通过拮抗的分割缺陷（PAR）蛋白复合物之间的平衡来指定分裂模式的不变谱系。通过在细胞分裂过程中使 PAR 蛋白的不均等遗传与命运决定因素的遗传分离，我们证明 PAR-2 和 PAR-6 之间平衡的变化足以使两个子细胞中的分裂模式从对称转变为不对称，反之亦然。所采用的分裂模式与细胞质命运决定因素、细胞大小不对称以及姐妹细胞之间的细胞周期不同步的不对称性无关。我们提出，PAR 蛋白之间的平衡代表了发育过程中指定两种分裂模式的内在自组织线索。
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