Department of Anatomy, University of California, San Francisco, CA 94158.
Department of Anatomy, University of California, San Francisco, CA 94158
Proc Natl Acad Sci U S A. 2021 Jul 13;118(28). doi: 10.1073/pnas.2105732118.
A remarkable molecular and functional heterogeneity of the primary sensory neurons and dorsal horn interneurons transmits pain- and or itch-relevant information, but the molecular signature of the projection neurons that convey the messages to the brain is unclear. Here, using retro-TRAP (translating ribosome affinity purification) and RNA sequencing, we reveal extensive molecular diversity of spino- and trigeminoparabrachial projection neurons. Among the many genes identified, we highlight distinct subsets of -, -, -, and -expressing projection neurons. By combining in situ hybridization of retrogradely labeled neurons with Fos-based assays, we also demonstrate significant functional heterogeneity, including both convergence and segregation of pain- and itch-provoking inputs into molecularly diverse subsets of NK1R- and non-NK1R-expressing projection neurons.
初级感觉神经元和背角中间神经元的显著分子和功能异质性传递疼痛和/或瘙痒相关信息，但将这些信息传递到大脑的投射神经元的分子特征尚不清楚。在这里，我们使用 retro-TRAP（翻译核糖体亲和纯化）和 RNA 测序，揭示了脊髓和三叉神经-臂旁投射神经元的广泛分子多样性。在鉴定的许多基因中，我们突出了表达 - 、 - 、 - 和 - 的投射神经元的不同亚群。通过将逆行标记神经元的原位杂交与基于 Fos 的测定相结合，我们还证明了显著的功能异质性，包括疼痛和瘙痒刺激输入在 NK1R 和非 NK1R 表达投射神经元的分子多样性亚群中的会聚和分离。
