Department of Oral and Maxillofacial Pathology and Oral Microbiology, SDM College of Dental Sciences and Hospital, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, Dharwad, India.
Department of Oral and Maxillofacial Pathology and Oral Microbiology, SDM College of Dental Sciences and Hospital, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, Dharwad, India,
Acta Cytol. 2021;65(5):361-367. doi: 10.1159/000517166. Epub 2021 Jul 8.
To determine the role and efficacy of fine needle aspiration cytology (FNAC) and cell block in diagnosis of jaw lesions and compare the agreement between FNAC and cell block to predict the diagnosis.
The sample comprised 51 cases, including 12 odontogenic keratocysts (OKCs), 8 ameloblastomas, 22 radicular cysts, 7 dentigerous cysts, and 1 each of intraosseous mucoepidermoid carcinoma (MEC) and adenomatoid odontogenic tumor (AOT). FNAC samples remaining after hematoxylin and eosin (H&E)-stained cytosmear diagnosis were centrifuged at 3,000 rpm for 10 min. The supernatant was discarded and sediment mixed with 2-3 mL alcohol and filtered. To this, 10% formalin was added, filtered, taken for routine processing, and stained with H&E. The result of FNAC smear and cell block was compared with histopathological diagnosis.
On cytological examination of the smears, 7 OKCs and 22 radicular cysts were diagnosed, whereas ameloblastomas, AOT, intraosseous MEC, and dentigerous cysts were not. This gave an agreement of 56.8% with the biopsy reports. Cell block sections stained with H&E of 12 OKCs, 22 radicular cysts, 1 MEC, and 3 cases of ameloblastoma offered a diagnosis in accordance with the biopsies giving an agreement of 74.5%, while dentigerous cyst and AOT failed to do so. In comparison with FNAC, additionally 5 cases of OKC and 1 of MEC could be detected, and in ameloblastoma, out of 8 cases, only 3 yielded a concordant diagnosis through the cell block technique.
In comparison with FNAC, the architectural pattern and the morphology of the cells were better preserved by the cell block technique. This substantiates that cell block could be used as an ancillary technique to aid in definitive diagnosis of head and neck swellings.
确定细针抽吸细胞学（FNAC）和细胞块在颌骨病变诊断中的作用和疗效，并比较 FNAC 和细胞块在预测诊断方面的一致性。
样本包括 51 例，包括 12 例牙源性角化囊肿（OKC）、8 例成釉细胞瘤、22 例根尖囊肿、7 例含牙囊肿和 1 例骨内黏液表皮样癌（MEC）和腺牙源性肿瘤（AOT）。在苏木精和伊红（H&E）染色细胞涂片诊断后，将 FNAC 样本以 3000rpm 离心 10 分钟。弃去上清液，将沉淀物与 2-3mL 酒精混合并过滤。向其中加入 10%福尔马林，过滤，进行常规处理，并进行 H&E 染色。比较 FNAC 涂片和细胞块的结果与组织病理学诊断。
在细胞学检查中，7 例 OKC 和 22 例根尖囊肿被诊断出来，而成釉细胞瘤、AOT、骨内 MEC 和含牙囊肿则未被诊断出来。这与活检报告的一致性为 56.8%。用 H&E 染色的 12 例 OKC、22 例根尖囊肿、1 例 MEC 和 3 例成釉细胞瘤的细胞块切片与活检结果一致，一致性为 74.5%，而含牙囊肿和 AOT 则不一致。与 FNAC 相比，还可以检测到 5 例 OKC 和 1 例 MEC，在成釉细胞瘤中，8 例中只有 3 例通过细胞块技术得出一致的诊断。
与 FNAC 相比，细胞块技术更好地保留了组织结构和细胞形态。这证明细胞块可以作为辅助技术，辅助头颈部肿块的明确诊断。
