慢性乙型肝炎病毒感染患者轻链库中体细胞突变水平较低。
Lower Somatic Mutation Levels in the Light-Chain Repertoires with Chronic HBV Infection.
作者信息
Hong Binbin, Liu Qiaoling, Li Qiulan, Su Lili, Wang Lizhi
机构信息
Central Laboratory, Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China.
Traditional Chinese Medicine Department, Rehabilitation Hospital, Nan'an, Quanzhou 362300, China.
出版信息
Evid Based Complement Alternat Med. 2021 Jun 7;2021:5525369. doi: 10.1155/2021/5525369. eCollection 2021.
To investigate the characteristics of the immunoglobulin light-chain repertoires with chronic HBV infection, the high-throughput sequencing and IMGT/HighV-QUEST were adapted to analyze the (IgK) and (IgL) light-chain repertoires from the inactive HBV carriers (IHB) and the healthy adults (HH). The comparative analysis revealed high similarity between the light-chain repertoires of the HBV carriers and the healthy adults. Nevertheless, the proportion of IGLV genes with ≥90% identity as the germline genes was higher in the IgL light-chain repertoire of the IHB library compared with that of HH library (74.6% 69.1%). Besides, the frequency of amino acid mutations in the CDR1 regions was significantly lower in the IgL light-chain repertoire of the IHB library than that of the HH library (65.52% 56.0%). These results suggested the lower somatic mutation level in the IgL repertoire of IHB library, which might indicate the biased selection of IGLV genes in the IgL repertoire with chronic HBV infection. These findings might lead to a better understanding of the characteristics of the light-chain repertoires of HBV chronically infected individuals.
为研究慢性乙型肝炎病毒（HBV）感染时免疫球蛋白轻链库的特征，采用高通量测序和IMGT/HighV-QUEST分析了非活动性HBV携带者（IHB）和健康成年人（HH）的免疫球蛋白κ链（IgK）和免疫球蛋白λ链（IgL）轻链库。比较分析显示，HBV携带者和健康成年人的IgL轻链库高度相似。然而，与HH文库相比，IHB文库的IgL轻链库中与种系基因一致性≥90%的IGLV基因比例更高（74.6%对69.1%）。此外，IHB文库的IgL轻链库中互补决定区1（CDR1）区域的氨基酸突变频率显著低于HH文库（65.52%对56.0%）。这些结果提示，IHB文库的IgL库中体细胞突变水平较低，这可能表明慢性HBV感染时IgL库中IGLV基因存在偏向性选择。这些发现可能有助于更好地理解慢性HBV感染个体轻链库的特征。
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