Li Gaoshang, Zhan Junqi, Hu Lingping, Yuan Chunhong, Ying Xiaoguo, Hu Yaqin
Institute of Food Engineering, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.
College of Food Science and Technology, Hainan Tropical Ocean University, Sanya, China.
J Food Biochem. 2022 Apr;46(4):e13853. doi: 10.1111/jfbc.13853. Epub 2021 Jul 8.
A novel antioxidant peptide EDEQKFWGK from porcine plasma hydrolysate (PPH) was separated by chromatography, HPLC, and identified by LC-MS/MS. Results showed that EDEQKFWGK had better antioxidant ability (Hydroxyl RAS 32.19%, ABTS RAS 92.93% and DPPH RAS 26.76%) compared with glutathione (30.11%, 82.01%, 26.44%) due to the presence of hydrophobic, aromatic acids (F, W) and acidic amino acids (E, D), decreasing ROS by providing hydrogen atom and chelating metal ions. Furthermore, the antioxidant properties of synthetic EDEQKFWGK still significant despite in vitro digestion because of the production of smaller active peptide. Additionally, it could increase SOD, CAT, GSH-Px to resist oxidative damage in HepG2 cells by inhibiting ROS (O , OH·), forming complexes to prevent OH· from destroying DNA and binding to ARE to promote antioxidase expression. Thereby, the novel peptide EDEQKFWGK from porcine plasma had much stable antioxidant properties and hade great potential in formulating functional foods. PRACTICAL APPLICATIONS: This research isolated a novel antioxidant peptide. Moreover, the antioxidant effects of peptide were confirmed under the in vitro digestion model and oxidative damage HepG2 cells model. The results showed the antioxidant peptide could play better effect after digestion and protect the cells from oxidative damage. These data could expand the sequence data of antioxidant peptides and promote the high-value utilization of PPH.
通过色谱法、高效液相色谱法从猪血浆水解物（PPH）中分离出一种新型抗氧化肽EDEQKFWGK，并通过液相色谱-串联质谱法进行鉴定。结果表明，与谷胱甘肽（30.11%、82.01%、26.44%）相比，EDEQKFWGK具有更好的抗氧化能力（羟基自由基清除率32.19%、ABTS自由基阳离子清除率92.93%、DPPH自由基清除率26.76%），这归因于其含有疏水、芳香酸（F、W）和酸性氨基酸（E、D），通过提供氢原子和螯合金属离子来降低活性氧。此外，尽管经过体外消化，合成的EDEQKFWGK仍具有显著的抗氧化性能，因为会产生较小的活性肽。此外，它可以通过抑制活性氧（O₂、OH·）来增加超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶，以抵抗HepG2细胞中的氧化损伤，形成复合物以防止OH·破坏DNA，并与抗氧化反应元件结合以促进抗氧化酶的表达。因此，来自猪血浆的新型肽EDEQKFWGK具有非常稳定的抗氧化性能，在功能性食品配方中具有巨大潜力。实际应用：本研究分离出一种新型抗氧化肽。此外肽的抗氧化作用在体外消化模型和氧化损伤HepG2细胞模型下得到了证实。结果表明抗氧化肽在消化后能发挥更好的效果，并保护细胞免受氧化损伤。这些数据可以扩展抗氧化肽的序列数据，并促进猪血浆水解物的高值利用。
