Wang Kai, Wu Pingxian, Wang Shujie, Ji Xiang, Chen Dong, Xiao Weihang, Gu Yiren, Zeng Yangshuang, Xu Xu, Tang Guoqing
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Sichuan Animal Science Academy, Chengdu, 610066, Sichuan, China.
Genome. 2021 Jul 9:1-8. doi: 10.1139/gen-2021-0006.
The Chinese Qingyu pig is a typical domestic fatty pig breed and an invaluable indigenous genetic resource in China. Compared with the Landrace pig, the Qingyu pig has unique meat characteristics, including muscle development, intramuscular fat, and other meat quality traits. At present, few studies have explored epigenetic differences due to DNA methylation between the Qingyu pig and the Landrace pig. In this study, 30 Qingyu pigs and 31 Landrace pigs were subjected to reduced representation bisulfite sequencing (RRBS). Genome-wide differential DNA methylation analysis was conducted. Six genomic regions, including regions on chromosome (SSC) 1: 266.09-274.23 Mb, SSC5: 0.88-10.68 Mb, SSC8: 41.23-48.51 Mb, SSC12: 45.43-54.38 Mb, SSC13: 202.15-207.95 Mb, and SSC14: 126.43-139.85 Mb, were regarded as key regions that may be associated with phenotypic differences between the Qingyu pig and the Landrace pig. Furthermore, according to further analysis, five differentially methylated genes (, , , , and ) were identified as key candidate genes that might be associated with meat characteristics. Our findings provide new insights into the differences in DNA methylation between the Qingyu pig and the Landrace pig. These results enrich the epigenetic research of the Chinese Qingyu pig.
中国青峪猪是典型的国内脂肪型猪品种，也是中国宝贵的本土遗传资源。与长白猪相比，青峪猪具有独特的肉质特性，包括肌肉发育、肌内脂肪和其他肉质性状。目前，很少有研究探讨青峪猪和长白猪之间由于DNA甲基化导致的表观遗传差异。在本研究中，对30头青峪猪和31头长白猪进行了简化代表性亚硫酸氢盐测序（RRBS）。进行了全基因组差异DNA甲基化分析。六个基因组区域，包括1号染色体（SSC）上266.09 - 274.23 Mb、5号染色体（SSC）上0.88 - 10.68 Mb、8号染色体（SSC）上41.23 - 48.51 Mb、12号染色体（SSC）上45.43 - 54.38 Mb、13号染色体（SSC）上202.15 - 207.95 Mb和14号染色体（SSC）上126.43 - 139.85 Mb的区域，被视为可能与青峪猪和长白猪表型差异相关的关键区域。此外，通过进一步分析，鉴定出五个差异甲基化基因（、、、和）为可能与肉质特性相关的关键候选基因。我们的研究结果为青峪猪和长白猪之间DNA甲基化差异提供了新的见解。这些结果丰富了中国青峪猪的表观遗传研究。
