高功率近共焦法布里-珀罗腔用于相衬电子显微镜。
High-power near-concentric Fabry-Perot cavity for phase contrast electron microscopy.
机构信息
Department of Physics, 366 Physics MS 7300, University of California-Berkeley, Berkeley, California 94720, USA.
California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, California 94720, USA.
出版信息
Rev Sci Instrum. 2021 May 1;92(5):053005. doi: 10.1063/5.0045496.
Transmission electron microscopy (TEM) of vitrified biological macromolecules (cryo-EM) is limited by the weak phase contrast signal that is available from such samples. Using a phase plate would thus substantially improve the signal-to-noise ratio. We have previously demonstrated the use of a high-power Fabry-Perot cavity as a phase plate for TEM. We now report improvements to our laser cavity that allow us to achieve record continuous wave intensities of over 450 GW/cm, sufficient to produce the optimal 90° phase shift for 300 keV electrons. In addition, we have performed the first cryo-EM reconstruction using a laser phase plate, demonstrating that the stability of this laser phase plate is sufficient for use during standard cryo-EM data collection.
冷冻电子显微镜（cryo-EM）中的生物大分子的传输电子显微镜（TEM）受到此类样品中可用的弱相位对比信号的限制。因此，使用相板会大大提高信号噪声比。我们之前已经证明了使用高功率法布里-珀罗腔作为 TEM 的相板。现在，我们报告了对激光腔的改进，这些改进使我们能够实现超过 450GW/cm 的创纪录连续波强度，足以产生 300keV 电子的最佳 90°相移。此外，我们还使用激光相板进行了首次 cryo-EM 重建，证明这种激光相板的稳定性足以用于标准 cryo-EM 数据采集。
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