Ministry of Education Key Laboratory of Protein Sciences, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center of Biological Structures, School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing 100084, China.
Ministry of Education Key Laboratory of Protein Sciences, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center of Biological Structures, School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing 100084, China.
Curr Opin Struct Biol. 2019 Oct;58:175-182. doi: 10.1016/j.sbi.2019.06.013. Epub 2019 Jul 30.
Not long after the invention of transmission electron microscope (TEM), phase plate was proposed as a novel electron-optical apparatus at the back-focal plane of the objective lens to modulate the magnified specimen images with enhanced contrast, especially in cryo-electron microscopy (cryo-EM) application of biological specimens. In the past two decades, novel phase plates of different kinds were designed and fabricated for cryo-EM application. Some of them such as the Volta phase plate have already been proved very useful in single particle cryo-EM and cryo-electron tomography (cryo-ET) analysis. In this review, we discuss the current progress, challenges and opportunities of cryo-EM with phase plate.
透射电子显微镜（TEM）发明后不久，在物镜的后焦平面上提出了相衬板作为一种新型的电子光学仪器，用于调制放大后的标本图像，以增强对比度，特别是在生物标本的冷冻电子显微镜（cryo-EM）应用中。在过去的二十年中，为 cryo-EM 应用设计和制造了新型的不同类型的相衬板。其中一些，如 Volta 相衬板，已经在单颗粒 cryo-EM 和 cryo-ET 分析中被证明非常有用。在这篇综述中，我们讨论了 cryo-EM 与相衬板的最新进展、挑战和机遇。
