State Key Laboratory of Agricultural Microbiology/Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, 430070, China.
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
J Integr Plant Biol. 2021 Oct;63(10):1801-1814. doi: 10.1111/jipb.13149. Epub 2021 Aug 1.
The post-translational modification lysine 2-hydroxyisobutyrylation (K ) plays an important role in gene transcription, metabolism, and enzymatic activity. K sites have been identified in rice (Oryza sativa). However, the K status of proteins in rice flowers during pathogen infection remains unclear. Here, we report a comprehensive identification of K -modified proteins in rice flowers, and the changes in these proteins during infection with the fungal pathogen Ustilaginoidea virens. By using a tandem mass tag-based quantitative proteomics approach, we identified 2,891 K sites on 964 proteins in rice flowers. Our data demonstrated that 2-hydroxyisobutyrylated proteins are involved in diverse biological processes. K levels were substantially reduced upon infection with U. virens. Chromatin immunoprecipitation polymerase chain reaction (PCR) and reverse transcription quantitative PCR analyses revealed that histone K is involved in the expression of disease-resistance genes. More importantly, most quantified sites on core histones H3 were downregulated upon U. virens infection. In addition, the histone deacetylases HDA705, HDA716, SRT1, and SRT2 are involved in the removal of K marks in rice. HDA705 was further confirmed to negatively regulate rice disease resistance to pathogens U. virens, Magnaporthe oryzae, and Xanthomonas oryzae pv. oryzae (Xoo). Our data suggest that U. virens could modulate K in rice flowers during infection.
翻译后修饰赖氨酸2-羟基异丁酰化（K）在基因转录、代谢和酶活性中发挥着重要作用。K位点已在水稻（Oryza sativa）中被鉴定出来。然而，在病原体感染期间水稻花中蛋白质的K状态仍不清楚。在此，我们报告了水稻花中K修饰蛋白质的全面鉴定，以及在感染真菌病原体稻曲病菌（Ustilaginoidea virens）期间这些蛋白质的变化。通过使用基于串联质量标签的定量蛋白质组学方法，我们在水稻花中的964种蛋白质上鉴定出2891个K位点。我们的数据表明，2-羟基异丁酰化蛋白质参与多种生物学过程。感染稻曲病菌后，K水平大幅降低。染色质免疫沉淀聚合酶链反应（PCR）和逆转录定量PCR分析表明，组蛋白K参与抗病基因的表达。更重要的是，在感染稻曲病菌后，核心组蛋白H3上的大多数定量位点下调。此外，组蛋白去乙酰化酶HDA705、HDA716、SRT1和SRT2参与水稻中K标记的去除。进一步证实HDA705负调控水稻对病原体稻曲病菌、稻瘟病菌（Magnaporthe oryzae）和水稻白叶枯病菌（Xanthomonas oryzae pv. oryzae，Xoo）的抗病性。我们的数据表明，稻曲病菌在感染期间可调节水稻花中的K。
