Mello M L, Raymundo H H
Histochemistry. 1977 Dec 7;54(3):219-23. doi: 10.1007/BF00492244.
Feulgen-DNA values were evaluated cytophotometrically in the Malpighian tubes of T. infestans at the nymphal, adult and ageing life periods. In all cases the total nuclear Feulgen-DNA contents were found to be distributed within the 32C and 64C classes. However, part of the Feulgen-DNA values is shifted to lower intervals in ageing insects, as compared with nymphal and adult individuals, promoted by decrease in Feulgen-DNA values of the euchromatins. Changed response to Feulgen reaction due to some aging-induced alteration in DNP complexes, and/or breaks and loss of DNA in the euchromatins, as part of the senescence process, are ascribed to be responsible for such phenomenon.
采用细胞光度法对侵染锥蝽若虫期、成虫期和衰老期的马尔皮基氏管中的福尔根 DNA 值进行了评估。在所有情况下,均发现细胞核福尔根 DNA 总含量分布在 32C 和 64C 类别范围内。然而,与若虫和成虫个体相比,衰老昆虫的部分福尔根 DNA 值向较低区间偏移,这是由常染色质的福尔根 DNA 值降低所致。由于衰老诱导的脱氧核糖核蛋白复合物的某些变化,和/或作为衰老过程一部分的常染色质中 DNA 的断裂和丢失,导致对福尔根反应的响应发生改变,被认为是造成这种现象的原因。