College of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China; College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Aug 1;1179:122851. doi: 10.1016/j.jchromb.2021.122851. Epub 2021 Jun 29.
Ti-based immobilized metal affinity chromatography (IMAC) nanomaterial has shown high potential in phosphoproteome mass-spectrometric (MS) analysis. However, the limited surface area and poor solubility will greatly restrict its use in phosphoproteome research. To overcome these two key drawbacks, a novel Ti-based IMAC nanomaterial was prepared by Ti-bonded β-cyclodextrin (β-CD) anchored on the surface of carbon nanotubes (CNTs) (denoted as COOH-CNTs-CD-Ti) and successfully applied as a biofunctional adsorbent for selectively enriching trace phosphopeptides. In the selective enrichment process, CNTs provided greater surface area for the absorption of phosphopeptides, while β-CD also offered a greater opportunity for the interaction between phosphopeptides and Ti. COOH-CNTs-CD-Ti with the aforementioned properities exhibited higher selectivity for phosphopeptides from the standard protein digests, the tryptic digests of nonfat milk and human serum, showing a great selective enrichment capability towards complex biological samples.
基于钛的固载金属亲和层析（IMAC）纳米材料在磷酸肽质谱（MS）分析中显示出巨大的潜力。然而，有限的比表面积和较差的溶解性将极大地限制其在磷酸肽组学研究中的应用。为了克服这两个关键缺陷，通过将 Ti 键合的β-环糊精（β-CD）锚定在碳纳米管（CNTs）表面上（表示为 COOH-CNTs-CD-Ti），制备了一种新型基于 Ti 的 IMAC 纳米材料，并成功地用作生物功能吸附剂，用于选择性富集痕量磷酸肽。在选择性富集过程中，CNTs 为磷酸肽的吸收提供了更大的表面积，而β-CD 也为磷酸肽与 Ti 之间的相互作用提供了更多的机会。具有上述特性的 COOH-CNTs-CD-Ti 对标准蛋白消化物、非脂乳的胰蛋白酶消化物和人血清中的磷酸肽表现出更高的选择性，对复杂的生物样品具有很强的选择性富集能力。
