National Engineering Laboratory of Biohydrometallurgy, GRINM Group Co., Ltd., Beijing, 100088, China; School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, Beijing, 100083, China; GRINM Resources and Environment Tech. Co., Ltd., Beijing, 101407, China; General Research Institute for Nonferrous Metals, Beijing, 100088, China.
College of Resource and Environmental Science, South-Central University for Nationalities, Wuhan, 430074, China.
Environ Res. 2021 Nov;202:111659. doi: 10.1016/j.envres.2021.111659. Epub 2021 Jul 9.
Electrolytic manganese residue (EMR) is a kind of solid waste with a high silicon content. Most of the silicon in EMR, however, exist in the state of SiO, which cannot be directly absorbed by plants. Currently, it is very challenge to recover the silicon from EMR. In this study, a preliminary screening of strains with silicon-activating ability was conducted, and four strains were screened out and isolated from the soil around the tailings pond of EMR. Then, single factor experiments were conducted to obtain the optimal growth conditions of the four strains, and the results indicated that the Ochrobactrum sp. T-07 had the best silicon-activating ability from EMR after nitrosoguanidine mutagenesis (Ochrobactrum sp. T-07-B). The available silicon (in terms of SiO) in the leaching solution was up to 123.88 mg L, which was significantly higher than that produced by Bacillus circulans and Paenibacillus mucilaginosus, the two commercial available pure culture strains. Results of direct/indirect contact experiments between Ochrobactrum sp. T-07-B and EMR revealed that bioleaching was promoted under the synergistic effect of bacteria growth on the surface of and metabolism within EMR. The newly isolated strains with silicon-activating effect are different from the existing-known silicate bacteria and may be used for more efficient silicon activation in silicate minerals.
电解锰渣（EMR）是一种高硅含量的固体废物。然而，EMR 中的大部分硅以 SiO 的形式存在，植物无法直接吸收。目前，从 EMR 中回收硅极具挑战性。本研究对具有硅激活能力的菌株进行了初步筛选，从 EMR 尾矿库周围的土壤中筛选并分离出了 4 株菌株。然后，通过单因素实验获得了 4 株菌的最佳生长条件，结果表明，经亚硝基胍诱变后的 Ochrobactrum sp. T-07（Ochrobactrum sp. T-07-B）对 EMR 具有最佳的硅激活能力。浸出液中的有效硅（以 SiO 计）高达 123.88 mg/L，明显高于两种市售的纯培养菌株环状芽孢杆菌和粘质沙雷氏菌。Ochrobactrum sp. T-07-B 与 EMR 之间的直接/间接接触实验结果表明，细菌在 EMR 表面生长和代谢的协同作用下促进了生物浸出。新分离的具有硅激活作用的菌株不同于现有的硅酸盐细菌，可能用于更有效地激活硅酸盐矿物中的硅。
