Institut National de la Transfusion Sanguine (INTS), Département d'études des Agents Transmissibles par le Sang, Centre National de Référence Risques Infectieux Transfusionnels, F-75015 Paris, France.
Institut National de la Transfusion Sanguine (INTS), Département d'études des Agents Transmissibles par le Sang, Centre National de Référence Risques Infectieux Transfusionnels, F-75015 Paris, France.
Infect Genet Evol. 2021 Oct;94:104995. doi: 10.1016/j.meegid.2021.104995. Epub 2021 Jul 9.
In the framework of a viral discovery research program using metagenomics, Human Pegivirus-1 reads (HPgV-1, formerly known as GBV-C) were detected in plasma pools of healthy blood donors from seven sub-Saharan African countries. For five of these countries, Mauritania, Mali, Niger, Burundi and Madagascar, no data about HPgV-1 genotypes was reported to date. To confirm our metagenomic findings and further investigate the genotype diversity and distribution of HPgV-1 in Africa, 400 blood donations from these five localities as well as from Cameroon, the Democratic Republic of Congo (DRC) and the Burkina Faso were screened with a RT-nested PCR targeting the viral 5'NCR region. Amplified products were sequenced, and the virus was genotyped by phylogenetic analysis. Out of the 400 plasma samples tested, 65 were positive for HPgV-1 RNA and 61 were successfully genotyped. Among these, 54 strains (88.5%) clustered with genotype 1, six (9.8%) with genotype 2 and one (1.6%) with genotype 5. Genotype 1 was observed in all countries studied, except in Madagascar, genotype 2 was detected in Mauritania and Madagascar, and genotype 5 in DRC. Overall, our results extend the geographic distribution of HPgV-1 in Africa and provide six additional nearly complete genomes. Considering that some HPgV-1 genotypes have been reported as potential predictive indicators of lower disease progression in HIV-1 infected subjects, further investigations should be conducted to better understand the positive impact, if any, of this virus.
在使用宏基因组学进行病毒发现研究计划的框架内，从七个撒哈拉以南非洲国家的健康献血者的血浆池中检测到了人类 Pegivirus-1 读码序列（HPgV-1，以前称为 GBV-C）。对于其中五个国家，毛里塔尼亚、马里、尼日尔、布隆迪和马达加斯加，迄今为止没有报告关于 HPgV-1 基因型的数据。为了确认我们的宏基因组学发现，并进一步研究非洲 HPgV-1 的基因型多样性和分布，我们用针对病毒 5'NCR 区域的 RT-巢式 PCR 筛选了来自这五个地区以及喀麦隆、刚果民主共和国和布基纳法索的 400 份献血。扩增产物进行测序，并通过系统发育分析对病毒进行基因分型。在测试的 400 个血浆样本中，有 65 个为 HPgV-1 RNA 阳性，61 个成功进行了基因分型。其中，54 株（88.5%）与基因型 1 聚类，6 株（9.8%）与基因型 2 聚类，1 株（1.6%）与基因型 5 聚类。除了马达加斯加外，所有研究国家都观察到基因型 1，在毛里塔尼亚和马达加斯加检测到基因型 2，在刚果民主共和国检测到基因型 5。总体而言，我们的结果扩展了非洲 HPgV-1 的地理分布，并提供了六个额外的几乎完整的基因组。考虑到一些 HPgV-1 基因型已被报道为 HIV-1 感染患者疾病进展较低的潜在预测指标，应进一步进行研究以更好地了解该病毒的积极影响（如果有的话）。
