Department of Botany, University of Science and Technology, Bannu, Pakistan.
Department of Botany, University of Science and Technology, Bannu, Pakistan/Department of Zoology, University of Science and Technology, Bannu, Pakistan.
Pak J Pharm Sci. 2021 Jan;34(1):151-156.
The present research study investigates the phytochemical and pharmacological importance of Bromus pectinatus. Qualitative phytochemical analysis of this plant was carried out to use standard method for the presence of various bioactive constituents. Results showed the ethanolic extract contain natural product such as steroids, alkaloids, tannins, coumarin, saponins, flavonoids and phenols. These compounds play a key role to reducing various disease and microbial inhibition. The ethanolic extract also showed the antimicrobial and antifugal activity against different pathogenic bacterial strains e.g Escherichia coli, Micrococus leutus, Protus vulgarus, and Kelebsela pneumona and three fungal strains Aspergillus fumigatus, Aspergillus flavous, Aspergillus niger. The antioxidant assay was performed as % inhibition of DPPH (1, 1-diphenyl-2-picryl-hydrazyl) free radicals. The plant extract has more antioxidant activity as compared to ascorbic acid. The maximum concentration (800µg/ml) is the most effective of all. The plant extract showed the high cytotoxicity activity against Brine shrimp. Moreover, the plant extract exhibited allelopathic effect on different growth parameters of wheat plant mostly at higher concentration. These results indicate that the BPEE have a potential broad-spectrum antimicrobial, cytotoxic, antioxidant and phytotoxic activity due to the presence of bioactive compounds.
本研究调查了多枝赖草的植物化学和药理学重要性。采用标准方法对该植物进行了定性植物化学分析，以确定各种生物活性成分的存在。结果表明，该植物的乙醇提取物含有天然产物，如甾体、生物碱、单宁、香豆素、皂苷、黄酮类和酚类。这些化合物在减少各种疾病和抑制微生物方面发挥着关键作用。乙醇提取物还对不同的病原菌菌株（如大肠杆菌、微球菌、普罗维库斯 vulgarus 和肺炎克雷伯菌）和三种真菌菌株（烟曲霉、黄曲霉和黑曲霉）表现出抗菌和抗真菌活性。抗氧化测定作为 DPPH（1,1-二苯基-2-苦基肼）自由基的抑制%进行。与抗坏血酸相比，植物提取物具有更高的抗氧化活性。最大浓度（800μg/ml）是最有效的。植物提取物对盐水虾表现出高细胞毒性活性。此外，植物提取物在较高浓度下对小麦植物的不同生长参数表现出化感作用。这些结果表明，由于存在生物活性化合物，BPEE 具有广谱的抗菌、细胞毒性、抗氧化和植物毒性活性。
