Krüger I, Ghussen F, Nagel K, Isselhard W
Chirurgischen Universitätsklinik Köln.
Z Exp Chir Transplant Kunstliche Organe. 1987;20(5):257-63.
A pelvic extremity of the rat was recirculatorily perfused by means of a self-developed perfusion equipment in a perfusion pressure of 65 +/- 6 mmHg and a blood temperature of 35 degrees C during one hour. Tissue specimens were taken away in the end of the perfusion and 3 weeks later and the metabolite contents was determined in comparison with control groups. No change of the metabolite status was provable by the perfusion. The technical practicability of the perfusion of rat extremities could be shown, therefore, contrary to hitherto existing assumptions.
使用自行研发的灌注设备,在65±6 mmHg的灌注压力和35摄氏度的血液温度下,对大鼠的盆腔肢体进行一小时的再循环灌注。在灌注结束时和3周后采集组织样本,并与对照组比较测定代谢物含量。灌注未显示代谢物状态有变化。因此,与迄今的现有假设相反,大鼠肢体灌注的技术可行性得到了证实。