Faillace Giulia Ramos, Caruso Paula Bacaicoa, Timmers Luis Fernando Saraiva Macedo, Favero Débora, Guzman Frank Lino, Rechenmacher Ciliana, de Oliveira-Busatto Luisa Abruzzi, de Souza Osmar Norberto, Bredemeier Christian, Bodanese-Zanettini Maria Helena
Programa de Pós-Graduação em Genética e Biologia Molecular and Instituto Nacional de Ciência e Tecnologia: Biotec Seca-Pragas, Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
Laboratório de Bioinformática, Modelagem e Simulação de Biossistemas (LABIO), Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
Front Genet. 2021 Jun 25;12:632685. doi: 10.3389/fgene.2021.632685. eCollection 2021.
Osmotins are multifunctional proteins belonging to the thaumatin-like family related to plant stress responses. To better understand the functions of soybean osmotins in drought stress response, the current study presents the characterisation of four previously described proteins and a novel putative soybean osmotin (GmOLPa-like). Gene and protein structure as well as gene expression analyses were conducted on different tissues and developmental stages of two soybean cultivars with varying dehydration sensitivities (BR16 and EMB48 are highly and slightly sensitive, respectively). The analysed osmotin sequences share the conserved amino acid signature and 3D structure of the thaumatin-like family. Some differences were observed in the conserved regions of protein sequences and in the electrostatic surface potential. P21-like present the most similar electrostatic potential to osmotins previously characterised as promoters of drought tolerance in and . Gene expression analysis indicated that soybean osmotins were differentially expressed in different organs (leaves and roots), developmental stages (R1 and V3), and cultivars in response to dehydration. In addition, under dehydration conditions, the highest level of gene expression was detected for GmOLPa-like and P21-like osmotins in the leaves and roots, respectively, of the less drought sensitive cultivar. Altogether, the results suggest an involvement of these genes in drought stress tolerance.
渗透素是属于类甜蛋白家族的多功能蛋白，与植物应激反应相关。为了更好地了解大豆渗透素在干旱胁迫响应中的功能，本研究对四种先前描述的蛋白和一种新的假定大豆渗透素（类GmOLPa）进行了表征。对两个脱水敏感性不同的大豆品种（BR16高度敏感，EMB48轻度敏感）的不同组织和发育阶段进行了基因和蛋白质结构以及基因表达分析。分析的渗透素序列具有类甜蛋白家族保守的氨基酸特征和三维结构。在蛋白质序列的保守区域和静电表面电位方面观察到一些差异。类P21与先前在[具体文献1]和[具体文献2]中被表征为耐旱促进剂的渗透素具有最相似的静电电位。基因表达分析表明，大豆渗透素在不同器官（叶和根）、发育阶段（R1和V3）以及不同品种中对脱水的反应存在差异表达。此外，在脱水条件下，在耐旱性较差的品种的叶和根中，分别检测到类GmOLPa和类P21渗透素的最高基因表达水平。总之，结果表明这些基因参与了干旱胁迫耐受性。
