用于同时检测大豆和小麦的三重PCR技术的开发。
Development of triplex PCR for simultaneous detection of soybean and wheat.
作者信息
Shin Jiyong, Kim Mi-Ju, Kim Hae-Yeong
机构信息
Department of Food Science and Biotechnology, Institute of Life Sciences and Resources, Kyung Hee University, Yongin, 17104 Republic of Korea.
出版信息
Food Sci Biotechnol. 2021 Jun 6;30(6):801-806. doi: 10.1007/s10068-021-00926-8. eCollection 2021 Jun.
There is a constant demand for an effective detection method of major allergens, such as soybean () and wheat (). In this study, a triplex polymerase chain reaction (PCR) method was developed for rapid detection of soybean and wheat in processed food products. This triplex PCR contains soybean- and wheat-specific primer pairs and universal primer pair for plant species. Each primer pair was applied to 22 different plant species and showed high specificity with no amplification in non-target species. The sensitivity of triplex PCR for soybean and wheat was 10 pg. The detection limit for soybean and wheat in pea mixture was 0.1%. The developed triplex PCR showed high sensitivity and specificity and was applied to 21 different commercial products. The results were in accordance with the label. Thus, this method is expected to be useful in preventing allergy-related issues via accurate labeling of major allergens in food.
对大豆（）和小麦（）等主要过敏原的有效检测方法一直有需求。在本研究中，开发了一种三重聚合酶链反应（PCR）方法，用于快速检测加工食品中的大豆和小麦。这种三重PCR包含大豆和小麦特异性引物对以及植物物种通用引物对。每个引物对应用于22种不同植物物种，在非目标物种中无扩增，显示出高特异性。三重PCR对大豆和小麦的灵敏度为10 pg。豌豆混合物中大豆和小麦的检测限为0.1%。所开发的三重PCR显示出高灵敏度和特异性，并应用于21种不同的商业产品。结果与标签一致。因此，该方法有望通过准确标注食品中的主要过敏原，在预防过敏相关问题方面发挥作用。
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