Guo Lijin, Chao Xiaohuan, Huang Weiling, Li Zhenhui, Luan Kang, Ye Mao, Zhang Siyu, Liu Manqing, Li Hongmei, Luo Wen, Nie Qinghua, Zhang Xiquan, Luo Qingbin
Guangdong Laboratory, Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, China.
College of Animal Science, South China Agricultural University, Guangzhou, China.
Front Cell Dev Biol. 2021 Jun 24;9:653798. doi: 10.3389/fcell.2021.653798. eCollection 2021.
Lipid biosynthesis is a complex process, which is regulated by multiple factors including lncRNA. However, the role of lncRNA in chicken abdominal fat accumulation is still unclear. In this research, we collected liver tissues from six high abdominal fat rate Sanhuang broilers and six low abdominal fat rate Sanhuang broilers to perform lncRNA sequencing and small RNA sequencing. A total of 2,265 lncRNAs, 245 miRNAs, and 5,315 mRNAs were differently expressed. Among of them, 1,136 differently expressed genes were enriched in the metabolic process. A total of 36 differently expressed genes, which were considered as differently expressed lncRNAs' targets, were enriched in the metabolic process. In addition, we also found out that eight differently expressed miRNAs could target 19 differently expressed genes. and were shared in a cis-regulatory network and a differently expressed miRNA target relationship network. LncRNA-FNIP2/miR-24-3p/ axis was considered as a potential candidate that may participate in lipid synthesis. Experimentally, the objective reality of lncRNA-FNIP2/miR-24-3p/ axis was clarified and the regulation effect of lncRNA-FNIP2/miR-24-3p/ axis on synthesis was validated. In brief, our study reveals a potential novel regulatory mechanism that lncRNA-FNIP2/miR-24-3p/ axis was considered as being involved in lipid synthesis during chicken adipogenesis in liver.
脂质生物合成是一个复杂的过程，受包括长链非编码RNA（lncRNA）在内的多种因素调控。然而，lncRNA在鸡腹部脂肪蓄积中的作用仍不清楚。在本研究中，我们采集了6只高腹脂率三黄肉鸡和6只低腹脂率三黄肉鸡的肝脏组织，进行lncRNA测序和小RNA测序。共鉴定出2265个lncRNA、245个miRNA和5315个mRNA差异表达。其中，1136个差异表达基因富集于代谢过程。共有36个差异表达基因被认为是差异表达lncRNA的靶标，也富集于代谢过程。此外，我们还发现8个差异表达的miRNA可靶向19个差异表达基因。 和 共享于一个顺式调控网络和一个差异表达的miRNA靶标关系网络。LncRNA-FNIP2/miR-24-3p/ 轴被认为是可能参与脂质合成的潜在候选者。通过实验，明确了lncRNA-FNIP2/miR-24-3p/ 轴的客观存在，并验证了lncRNA-FNIP2/miR-24-3p/ 轴对脂质合成的调控作用。简而言之，我们的研究揭示了一种潜在的新调控机制，即lncRNA-FNIP2/miR-24-3p/ 轴参与鸡肝脏脂肪生成过程中的脂质合成。
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