Chao Xiaohuan, Guo Lijin, Wang Qi, Huang Weiling, Liu Manqing, Luan Kang, Jiang Jinqi, Lin Shudai, Nie Qinghua, Luo Wen, Zhang Xiquan, Luo Qingbin
Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, China.
Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China.
Front Cell Dev Biol. 2020 Dec 21;8:595637. doi: 10.3389/fcell.2020.595637. eCollection 2020.
To explore the regulatory mechanism of abdominal fat deposition in broilers, 100-day-old Sanhuang chickens ( = 12) were divided into high-fat and low-fat groups, according to the abdominal fat ratio size. Total RNA isolated from the 12 abdominal fat tissues was used for miRNA and mRNA sequencing. Results of miRNA and mRNA sequencing revealed that miR-429-3p was highly expressed in high-fat chicken whereas expression was downregulated. Further, we determined that miR-429-3p promoted preadipocyte proliferation and differentiation, whereas exerted an opposite effect. Notably, we found that the miR-429-3p/ axis facilitated PPARγ pathway activation, which is closely associated with the progression of adipogenesis. In conclusion, our results provide evidence that a novel miR-429-3p/ axis is involved in the regulation of adipogenesis, which may have a guiding role in the improvement of breeding for abdominal fat traits in broiler chickens.
为探究肉鸡腹部脂肪沉积的调控机制，将100日龄的三黄鸡（ = 12）按腹部脂肪比例大小分为高脂组和低脂组。从12个腹部脂肪组织中分离出的总RNA用于miRNA和mRNA测序。miRNA和mRNA测序结果显示，miR-429-3p在高脂鸡中高表达，而 表达下调。此外，我们确定miR-429-3p促进前脂肪细胞增殖和分化，而 发挥相反作用。值得注意的是，我们发现miR-429-3p/ 轴促进PPARγ途径激活，这与脂肪生成的进展密切相关。总之，我们的结果提供了证据，表明新的miR-429-3p/ 轴参与脂肪生成的调控，这可能对改善肉鸡腹部脂肪性状的育种具有指导作用。
