miR-429-3p/轴通过PPARγ途径促进鸡腹部脂肪沉积。

miR-429-3p/ Axis Promotes Chicken Abdominal Fat Deposition via PPARγ Pathway.

作者信息

Chao Xiaohuan, Guo Lijin, Wang Qi, Huang Weiling, Liu Manqing, Luan Kang, Jiang Jinqi, Lin Shudai, Nie Qinghua, Luo Wen, Zhang Xiquan, Luo Qingbin

机构信息

Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, China.

Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China.

出版信息

Front Cell Dev Biol. 2020 Dec 21;8:595637. doi: 10.3389/fcell.2020.595637. eCollection 2020.

To explore the regulatory mechanism of abdominal fat deposition in broilers, 100-day-old Sanhuang chickens ( = 12) were divided into high-fat and low-fat groups, according to the abdominal fat ratio size. Total RNA isolated from the 12 abdominal fat tissues was used for miRNA and mRNA sequencing. Results of miRNA and mRNA sequencing revealed that miR-429-3p was highly expressed in high-fat chicken whereas expression was downregulated. Further, we determined that miR-429-3p promoted preadipocyte proliferation and differentiation, whereas exerted an opposite effect. Notably, we found that the miR-429-3p/ axis facilitated PPARγ pathway activation, which is closely associated with the progression of adipogenesis. In conclusion, our results provide evidence that a novel miR-429-3p/ axis is involved in the regulation of adipogenesis, which may have a guiding role in the improvement of breeding for abdominal fat traits in broiler chickens.