Yeh Y C, Tessman I
J Biol Chem. 1978 Mar 10;253(5):1323-4.
Infection of Escherichia coli with phage T4 induces a large increase in ribonucleotide reductase activity. We show that hydroxyurea inhibits T4-induced CDP, ADP, UDP, and GDP reductase activities in vitro. Moreover, there are significant differences in the degree of inhibition of each ribonucleotide reductase activity. The reductase activities for CDP and ADP are more sensitive to hydroxyurea than those for UDP and GDP, particularly at high hydroxyurea molarities. As little as 5 x 10(-4)M hydroxyurea lowers CDP and ADP reductase activities to 25 to 30% whereas as much as 0.5 M hydroxyurea is needed to lower UDP and GDP reductase activities to 50%.
用噬菌体T4感染大肠杆菌会导致核糖核苷酸还原酶活性大幅增加。我们发现，羟基脲在体外可抑制T4诱导的CDP、ADP、UDP和GDP还原酶活性。此外，每种核糖核苷酸还原酶活性的抑制程度存在显著差异。CDP和ADP的还原酶活性比UDP和GDP的还原酶活性对羟基脲更敏感，尤其是在高羟基脲摩尔浓度下。低至5×10⁻⁴M的羟基脲可将CDP和ADP还原酶活性降低至25%至30%，而高达0.5M的羟基脲才能将UDP和GDP还原酶活性降低至50%。
