Department of Oncology, Medical University of Bialystok, Bialystok, Poland.
Department of Clinical Oncology, Comprehensive Cancer Center of Bialystok, Bialystok, Poland.
Pathol Oncol Res. 2021 Mar 31;27:605017. doi: 10.3389/pore.2021.605017. eCollection 2021.
Brain gliomas are characterized by remarkably intense invasive growth and the ability to create new blood vessels. Angiogenesis is a key process in the progression of these tumors. Coagulation and fibrinolysis factors play a role in promoting angiogenesis. The aim of the study was to evaluate the expression of proangiogenic proteins (VEGF and bFGF) and hemostatic proteins (TF, fibrinogen, fibrin, D-dimers) associated with neoplastic cells and vascular endothelial cells in brain gliomas of various degrees of malignancy. Immunohistochemical tests were performed using the ABC method with the use of mono- and polyclonal antibodies. The obtained results indicated that both neoplastic cells and vascular endothelial cells in gliomas of various degrees of malignancy are characterized by heterogeneous expression of proteins of the hemostatic system and angiogenesis markers. The strongest expression of proangiogenic factors and procoagulant factors was demonstrated in gliomas of higher-grade malignancy.
脑胶质瘤的特点是显著的侵袭性生长和生成新血管的能力。血管生成是这些肿瘤进展的关键过程。凝血和纤溶因子在促进血管生成中起作用。本研究的目的是评估与肿瘤细胞和血管内皮细胞相关的促血管生成蛋白（VEGF 和 bFGF）和止血蛋白（TF、纤维蛋白原、纤维蛋白、D-二聚体）在不同恶性程度脑胶质瘤中的表达。使用 ABC 法和单克隆和多克隆抗体进行免疫组织化学测试。结果表明，不同恶性程度的胶质瘤中的肿瘤细胞和血管内皮细胞的止血系统和血管生成标志物的表达均具有异质性。在恶性程度较高的胶质瘤中，促血管生成因子和促凝因子的表达最强。
