酮亚胺环：溶液中和聚丙烯酰胺凝胶上酶活性的有效检测剂。L-氨基酸氧化酶、谷氨酰胺转氨酶、泛硫乙胺酶和酰基转移酶I的检测。
Ketimine rings: useful detectors of enzymatic activities in solution and on polyacrylamide gel. Detection of L-amino acid oxidase, glutamine transaminase, pantetheinase, and acylase I.
作者信息
Ricci G, Caccuri A M, Lo Bello M, Solinas S P, Nardini M
机构信息
Institute of Biochemical Sciences, University of Chieti, Rome, Italy.
出版信息
Anal Biochem. 1987 Sep;165(2):356-61. doi: 10.1016/0003-2697(87)90281-8.
A simple procedure has been developed for the detection of L-amino acid oxidase, glutamine transaminase, pantetheinase, and acylase I in solution and on polyacrylamide gels. The method is based on the strong absorbance at 296 nm of some ketimine rings which can be directly produced by the enzymatic reaction or formed by the reaction of the enzymatic product with 3-bromopyruvate. The procedure allows one to visualize up to about 1-10 mU of enzyme.
已开发出一种简单方法，用于检测溶液和聚丙烯酰胺凝胶中的L-氨基酸氧化酶、谷氨酰胺转氨酶、泛解酸酶和酰基转移酶I。该方法基于某些酮亚胺环在296nm处有强吸光度，这些酮亚胺环可由酶促反应直接产生，或由酶促产物与3-溴丙酮酸反应形成。该方法可使低至约1-10mU的酶可视化。
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