Fernandez J G, de Paz P, Chamorro C A
Departamento de Biología Celular y Anatomía, Universidad de León, España.
Anat Rec. 1987 Nov;219(3):296-303. doi: 10.1002/ar.1092190310.
We have analyzed the effects of colchicine on the cell shapes in chick neuroepithelium. Cell shapes were ascertained by the position of the nucleus in plastic serial sections. We tested three colchicine doses (5 X 10(-5) M, 5 X 10(-6) M, and 5 X 10(-7) M) by two experimental treatments (in ovo and in vitro). Colchicine treatment in vitro is always effective in depolymerizing microtubules of neuroepithelial cells and reduces the percentages of wedge-shaped cells in the median area of neuroepithelium. The same effect can be observed when the embryos are treated with 5 X 10(-5) M or 5 X 10(-6) M colchicine in ovo. A concentration of colchicine of 5 X 10(-7) M in ovo cannot disrupt microtubules in stage 8 and stage 10 embryos, and the percentage of wedge-shaped cells is the same as that of the untreated cells. In stage 6 embryos this colchicine dose effects the microtubules and the percentages of wedge-shaped cells. These facts are interpreted in respect to variations in microtubular resistance to microtubular-disrupting agents that are shown by the neuroepithelial cells from different developmental stages.
我们分析了秋水仙碱对鸡神经上皮细胞形态的影响。通过细胞核在塑料连续切片中的位置来确定细胞形态。我们通过两种实验处理方式（在胚胎内和体外）测试了三种秋水仙碱剂量（5×10⁻⁵ M、5×10⁻⁶ M和5×10⁻⁷ M）。体外秋水仙碱处理总能有效地使神经上皮细胞的微管解聚，并降低神经上皮中间区域楔形细胞的百分比。当在胚胎内用5×10⁻⁵ M或5×10⁻⁶ M秋水仙碱处理胚胎时，也能观察到相同的效果。胚胎内5×10⁻⁷ M的秋水仙碱浓度在第8阶段和第10阶段胚胎中无法破坏微管，楔形细胞的百分比与未处理细胞相同。在第6阶段胚胎中，这种秋水仙碱剂量会影响微管和楔形细胞的百分比。这些事实是根据不同发育阶段的神经上皮细胞对微管破坏剂的微管抗性变化来解释的。
