Sandru G, Sandru L
J Immunol Methods. 1978;19(2-3):293-8. doi: 10.1016/0022-1759(78)90189-8.
A new in vitro method for MIF assay has been developed using cells contained in clotted plasma droplets. It gives results concordant with the capillary tube method for H37 Ra Mycobacteria sensitized mice and PPD sensitized healthy persons. The technique is applicable to any migratory cell population and requires only a small number of cells for performance. It is technically simple, not time consuming and practicable both in patients and experimental animals.
一种新的用于巨噬细胞移动抑制因子(MIF)检测的体外方法已被开发出来,该方法使用凝结血浆液滴中所含的细胞。对于用H37 Ra分枝杆菌致敏的小鼠和用纯化蛋白衍生物(PPD)致敏的健康人,其检测结果与毛细管法一致。该技术适用于任何迁移细胞群体,并且只需要少量细胞即可进行检测。它在技术上简单,不耗时,在患者和实验动物中均可行。