Sidhu H, Gupta R, Thind S K, Nath R
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Ann Nutr Metab. 1987;31(6):354-61. doi: 10.1159/000177294.
Male weanling rats were maintained on a thiamine-deficient diet for 4 weeks, and compared with ad libitum and pair-fed controls. Thiamine deficiency led to slow growth and finally a decrease in body weight. Liver and kidney weights of the deficient rats were low, but appropriate to the body weight. Thiamine deficiency also caused a significant decrease in erythrocyte transketolase levels. The decarboxylation of glyoxylate both via the glyoxylate oxidation cycle and alpha-ketoglutarate:glyoxylate (alpha-KG:GA) carboligase was significantly lower in the liver and kidney mitochondria, leading to accumulation of glyoxylate in the tissues and its excretion in the urine. Part of the accumulated glyoxylate is converted to oxalate, causing hyperoxaluria.
雄性断乳大鼠维持4周硫胺素缺乏饮食，并与自由采食和配对喂养的对照组进行比较。硫胺素缺乏导致生长缓慢，最终体重下降。缺乏硫胺素的大鼠肝脏和肾脏重量较低，但与体重相适应。硫胺素缺乏还导致红细胞转酮醇酶水平显著降低。肝脏和肾脏线粒体中通过乙醛酸氧化循环以及α-酮戊二酸：乙醛酸（α-KG：GA）羧化酶途径的乙醛酸脱羧作用均显著降低，导致组织中乙醛酸积累并经尿液排泄。部分积累的乙醛酸转化为草酸盐，导致高草酸尿症。
