Pinchasov Y, Nir I, Nitsan Z
Department of Animal Science, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Ann Nutr Metab. 1987;31(6):362-6. doi: 10.1159/000177295.
The effect of intermittent feeding (every 2nd day) on in vitro muscle protein synthesis and degradation was studied in adapted and nonadapted broiler chicks. Protein synthesis, estimated by in vitro incorporation of L-[14C]-tyrosine into muscle protein, was higher in the intermittently fed birds than that in ad libitum fed counterparts or nonadapted birds. In chicks exposed to one 24-hour cycle of food deprivation and restoration, protein synthesis was slightly depressed (nonsignificant). Protein degradation, estimated by free tyrosine released from muscle into the medium, decreased after 24 h of food deprivation and increased after 24 h of food restoration, to the same extent in both adapted and nonadapted birds.
在适应性和非适应性肉鸡雏鸡中研究了间歇性喂食（每隔一天）对体外肌肉蛋白质合成与降解的影响。通过体外将L-[14C]-酪氨酸掺入肌肉蛋白来估计蛋白质合成，间歇性喂食的鸡的蛋白质合成高于自由采食的同龄鸡或非适应性鸡。在经历一个24小时禁食和恢复周期的雏鸡中，蛋白质合成略有下降（不显著）。通过从肌肉释放到培养基中的游离酪氨酸来估计蛋白质降解，禁食24小时后蛋白质降解减少，恢复进食24小时后增加，适应性和非适应性鸡的变化程度相同。
