配体结合稳定端粒 G-四链体增加对 S1 核酸酶的敏感性。
Stabilization of telomeric G-quadruplex by ligand binding increases susceptibility to S1 nuclease.
机构信息
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
出版信息
Chem Commun (Camb). 2021 Jul 28;57(59):7236-7239. doi: 10.1039/D1CC03294A. Epub 2021 Jul 15.
The extent of thermodynamic stabilization of telomeric G-quadruplex (G4) by isomers of G4 ligand L2H2-6OTD, a telomestatin analog, is inversely correlated with susceptibility to S1 nuclease. L2H2-6OTD facilitated the S1 nuclease activities through the base flipping in G4, unlike the conventional role of G4 ligands which inhibit the protein binding to DNA/RNA upon ligand interactions.
端粒 G-四链体 (G4) 由端粒酶抑制剂类似物 L2H2-6OTD 的异构体稳定的程度与对 S1 核酸酶的易感性成反比。与传统的 G4 配体在配体相互作用时抑制蛋白与 DNA/RNA 结合的作用相反，L2H2-6OTD 通过 G4 中的碱基翻转促进了 S1 核酸酶的活性。
Zotero 插件