Kaneko S, Sato H, Kobanawa K, Oshio S, Kobayashi T, Iizuka R
Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan.
Arch Androl. 1987;19(1):75-84. doi: 10.3109/01485018708986803.
To increase the fertilizability of sperm for use in artificial insemination with husband's semen, it is effective to concentrate progressively motile sperm from whole ejaculated semen. The simple procedure, a continuous-step density gradient technique, was developed to selectively concentrate progressively motile sperm. The ejaculated semen was placed on 6.0 ml of 80% Percoll solution, and the density gradient was formed by mixing the semen and Percoll with an L-shaped rod for two or three strokes. After centrifugation at 400 x g for 30 min, progressively motile sperm were concentrated in the sediment, whereas the immotile sperm and other types of cells remained in the upper part of the density gradient. In most specimens sperm motility was improved by more than 80% regardless of the original motility. Thus, the fertility index (sperm concentration/ml x percentage of motility x 10(-8)). was increased to 7.7 +/- 3.7 times (n = 15).
为提高用于丈夫精液人工授精的精子受精能力，逐步从全份射出精液中浓缩具有前向运动能力的精子是有效的。已开发出一种简单程序，即连续梯度密度技术，用于选择性地浓缩具有前向运动能力的精子。将射出的精液置于6.0毫升80%的 Percoll 溶液上，用L形棒将精液和 Percoll 混合两到三下形成密度梯度。以400×g 离心30分钟后，具有前向运动能力的精子集中在沉淀物中，而不活动的精子和其他类型的细胞留在密度梯度的上部。在大多数标本中，无论原始运动能力如何，精子运动能力均提高了80%以上。因此，生育指数（每毫升精子浓度×运动能力百分比×10⁻⁸）提高到了7.7±3.7倍（n = 15）。
