用于检测绵羊伪结核棒状杆菌感染的酶联免疫吸附测定法的评估
Evaluation of an enzyme-linked immunosorbent assay for the detection of Corynebacterium pseudotuberculosis infection in sheep.
作者信息
Sutherland S S, Ellis T M, Mercy A R, Paton M, Middleton H
机构信息
Department of Agriculture, South Perth, Western Australia.
出版信息
Aust Vet J. 1987 Sep;64(9):263-6. doi: 10.1111/j.1751-0813.1987.tb15953.x.
Two enzyme-linked immunosorbent assays to measure antibody to the cell wall antigen (C-ELISA) and toxin antigen (T-ELISA) of C. pseudotuberculosis were evaluated on serum from 6 separate groups of sheep. For sheep naturally infected with C. pseudotuberculosis the sensitivity and specificity of the C-ELISA was 76% and 73% respectively and for the T-ELISA 67% and 77% respectively. For sheep slaughtered one year after artificial infection the sensitivity of both tests was greater than or equal to 83% and the specificity greater than or equal to 72%. For sheep slaughtered 4 months after artificial infection the specificity of both tests was less than 30% while the mean sensitivity was 85%. The C-ELISA in conjunction with the T-ELISA detected 92% of sheep with lung lesions.
对来自6个不同绵羊组的血清，评估了两种用于检测伪结核棒状杆菌细胞壁抗原抗体（C-ELISA）和毒素抗原抗体（T-ELISA）的酶联免疫吸附测定。对于自然感染伪结核棒状杆菌的绵羊，C-ELISA的敏感性和特异性分别为76%和73%，T-ELISA的敏感性和特异性分别为67%和77%。对于人工感染一年后宰杀的绵羊，两种检测方法的敏感性均大于或等于83%，特异性大于或等于72%。对于人工感染4个月后宰杀的绵羊，两种检测方法的特异性均小于30%，而平均敏感性为85%。C-ELISA与T-ELISA联合检测出92%有肺部病变的绵羊。
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