Department of Chemistry, University of Warwick, Coventry, West Midlands CV4 7AL, United Kingdom.
Chemical Development, Pharmaceutical Technology & Development, Operations, AstraZeneca, Macclesfield SK10 2NA, United Kingdom.
J Am Soc Mass Spectrom. 2021 Aug 4;32(8):2153-2161. doi: 10.1021/jasms.1c00106. Epub 2021 Jul 15.
Due to the natural dispersity that is present in synthetic polymers, an added complexity is always present in the analysis of polymeric species. Tandem mass spectrometry analysis requires the isolation of individual precursors before a fragmentation event to allow the unambiguous characterization of these species and is not viable at certain levels of complexity due to achievable isolation widths. Two-dimensional mass spectrometry (2DMS) fragments ions and correlates fragments with their corresponding precursors without the need for isolation. In this study, 2DMS electron capture dissociation (ECD) fragmentation of a polyoxazoline and polyacrylamide species was carried out, resulting in the analysis of byproducts and individual polymer species without the use of chromatographic techniques. This study shows that 2DMS ECD is a powerful tool for the analysis of polyacrylamide and polyoxazoline species and offers a new dimension in the characterization of polymers.
由于合成聚合物中存在天然的分散性，因此在分析聚合物种时总是存在额外的复杂性。串联质谱分析需要在碎片化事件之前分离单个前体，以允许对这些物质进行明确的特征描述，但由于可实现的分离宽度，在某些复杂程度下是不可行的。二维质谱（2DMS）无需分离即可对离子进行碎裂，并将碎片与其相应的前体相关联。在这项研究中，对聚恶唑啉和聚丙烯酰胺物质进行了二维质谱电子俘获解离（ECD）碎裂，无需使用色谱技术即可分析副产物和单个聚合物物质。本研究表明，2DMS ECD 是分析聚丙烯酰胺和聚恶唑啉物质的有力工具，并为聚合物的特征描述提供了新的维度。
