Waris T, Lähteenmäki T, Hukki J, von Smitten K, Kyösola K
Department of Surgery, Oulu University Central Hospital, Finland.
Basic Res Cardiol. 1987 Sep-Oct;82(5):445-53. doi: 10.1007/BF01907092.
Right atrial biopsies from rat and human hearts were studied using combined methods for the demonstration of glyoxylic acid-induced fluorescence (GIF) of catecholamines and acetylcholinesterase (AChE) reactions in the same specimens. In specimens from the rat heart, the GIF and AChE reactions were performed either simultaneously or consecutively. In biopsies of the human right atrium, obtained at right atria cannulation during open-heart surgery, the reactions were performed consecutively. It was found that in both rat and human atria the adrenergic nerves and AChE reactive nerves, which are probably cholinergic, run partly separately and partly together. In the rat atrial specimens, close relations between adrenergic nerves and clusters of AChE reactive cells were observed. In addition, clusters of fluorescent cells were observed in the vicinity of AChE reactive nerve bundles. This indicates that there may be several possible means of peripheral interaction between the intrinsic adrenergic and cholinergic systems of the rat heart.
采用联合方法对大鼠和人类心脏的右心房活检组织进行研究，以在同一样本中同时显示儿茶酚胺的乙醛酸诱导荧光（GIF）和乙酰胆碱酯酶（AChE）反应。在大鼠心脏的样本中，GIF和AChE反应可同时或连续进行。在心脏直视手术期间右心房插管时获取的人类右心房活检组织中，反应是连续进行的。结果发现，在大鼠和人类心房中，肾上腺素能神经和可能为胆碱能的AChE反应性神经部分分开走行，部分共同走行。在大鼠心房样本中，观察到肾上腺素能神经与AChE反应性细胞簇之间存在密切关系。此外，在AChE反应性神经束附近观察到荧光细胞簇。这表明大鼠心脏内在肾上腺素能和胆碱能系统之间可能存在多种外周相互作用方式。
