在拉伸标本中同时显示胆碱酯酶和乙醛酸诱导的儿茶酚胺荧光。

Simultaneous demonstration of cholinesterases and glyoxylic acid-induced fluorescence of catecholamines in stretch preparations.

作者信息

Waris T, Rechardt L, Partanen S

出版信息

Acta Histochem. 1977;58(2):194-8. doi: 10.1016/S0065-1281(77)80128-1.

Abstract

A combined simultaneous method to demonstrate adrenergic nerves using glyoxylic acid-induced fluorescence and nerves showing cholinesterase activity using the thiocholine technique is described in whole-mount preparations. The subcutaneous fascia and the right atrium of the heart of the rat and guinea-pig were used as tissue specimens, and the innervation patterns of adrenergic and cholinergic nerves were demonstrated in UV and transmitted light. Technical points and the limitations of the method are discussed.

摘要

本文描述了一种在整装标本中使用乙醛酸诱导荧光来显示肾上腺素能神经以及使用硫代胆碱技术来显示胆碱酯酶活性神经的联合同步方法。将大鼠和豚鼠的皮下筋膜及心脏右心房用作组织标本，并在紫外光和透射光下显示肾上腺素能和胆碱能神经的支配模式。讨论了该方法的技术要点及局限性。