Muxfeldt M, Schaper W
Max Planck Institute, Department of Experimental Cardiology, Bad Nauheim, FRG.
Basic Res Cardiol. 1987 Sep-Oct;82(5):486-92. doi: 10.1007/BF01907096.
We isolated and purified xanthine oxidase by Sephadex gel filtration and assayed the activity of the enzyme by urate production which we measured by HPLC and UV absorption at 290 nm. We applied this method to extracts of liver and heart of rats, guinea pigs, rabbits, and pigs and to heart of dogs and humans. We found that pig hearts do not exhibit xanthine oxidase activity and that human and rabbit hearts produced small amounts of urate only after hours of incubation. We conclude that xanthine oxidase does probably not play an important part in the mechanisms leading to myocardial infarction using the free radical generating pathway, because it is absent in one species (pig) and barely detectable in two others (rabbit and man) that are known for their rapid and complete infarction following acute coronary occlusion.
我们通过葡聚糖凝胶过滤法分离并纯化了黄嘌呤氧化酶，并通过尿酸生成来测定该酶的活性，我们使用高效液相色谱法和在290nm处的紫外吸收来测量尿酸生成。我们将此方法应用于大鼠、豚鼠、兔子和猪的肝脏和心脏提取物以及狗和人类的心脏。我们发现猪心脏不表现出黄嘌呤氧化酶活性，而人类和兔子的心脏仅在孵育数小时后才产生少量尿酸。我们得出结论，黄嘌呤氧化酶可能在通过自由基生成途径导致心肌梗死的机制中不起重要作用，因为在一个物种（猪）中不存在，而在另外两个物种（兔子和人类）中几乎检测不到，而这两个物种以急性冠状动脉闭塞后快速且完全梗死而闻名。
