Snow A D, Kisilevsky R, Stephens C, Anastassiades T
Department of Pathology, Queen's University, Kingston, Ontario, Canada.
Biomed Biochim Acta. 1987;46(7):537-46.
It has frequently been suggested that there may normally be small quantities of heparin in human blood, but amounts so small that their direct demonstration is not possible by available methods [1-4]. In this communication, we provide direct electrophoretic visualization that heparin or a closely related substance, is present in normal human plasma. After isolating glycosaminoglycans (GAGs) from 2-3 mls of plasma, a modification of the discontinuous high voltage electrophoretic method developed by Cappelletti et al. was used to successfully separate and identify the GAGs present in six normal adults (4 men and 2 women). In each case, in addition to chondroitin-4-SO4, chondroitin-6-SO4, and heparan-SO4, we were able to show the presence of a band which corresponds to that of heparin.
人们经常认为，人体血液中通常可能含有少量肝素，但含量极少，以至于现有方法无法直接证明其存在[1-4]。在本通讯中，我们提供了直接的电泳可视化结果，表明正常人血浆中存在肝素或与之密切相关的物质。从2-3毫升血浆中分离出糖胺聚糖（GAGs）后，采用Cappelletti等人开发的不连续高压电泳方法的改进方法，成功分离并鉴定了6名正常成年人（4名男性和2名女性）血浆中存在的GAGs。在每种情况下，除了硫酸软骨素-4、硫酸软骨素-6和硫酸乙酰肝素外，我们还能够显示出一条与肝素相对应的条带。
