Liljequist S, Culp S, Tabakoff B
Laboratory for Studies of Neuroadaptive Processes, NIAAA, Bethesda, MD 20892.
Alcohol Alcohol Suppl. 1987;1:653-6.
The effect of ethanol in vitro on the binding of 35S-t-butylbicyclophosphorothionate to brain membranes was examined. Ethanol inhibited basal binding of 35S-TBPS both in potassium bromide- (KBr)- and potassium chloride (KCl)-containing assay media. Ethanol was, however, considerably weaker in reducing 35S-TBPS binding in buffers with 100 mM KCl. Ethanol had no effect on the inhibition of 35S-TBPS binding produced by GABA and pentobarbital in the presence of KBr or KCl, whereas it clearly altered the enhancement of 35S-TBPS binding produced by these drugs when the assay was carried out in buffers containing KCl.
研究了乙醇在体外对35S-叔丁基双环磷硫代酸酯与脑膜结合的影响。在含有溴化钾（KBr）和氯化钾（KCl）的测定介质中，乙醇均抑制35S-TBPS的基础结合。然而，在含有100 mM KCl的缓冲液中，乙醇降低35S-TBPS结合的能力要弱得多。在存在KBr或KCl的情况下，乙醇对GABA和戊巴比妥产生的35S-TBPS结合抑制没有影响，而当在含有KCl的缓冲液中进行测定时，乙醇明显改变了这些药物产生的35S-TBPS结合增强作用。
