Department of General Surgery, Beijing YouAn Hospital, Capital Medical University, Beijing, China.
J BUON. 2021 May-Jun;26(3):875-881.
We aimed to investigate the effect of celecoxib on rats with liver cancer through the extracellular signal-regulated kinase (ERK)/c-Jun N-terminal kinase (JNK)/p38 pathway.
Sprague-Dawley rats (n=36) were divided into 3 groups (n=12 per group) randomly. In model group, the liver cancer model was established, and normal saline was intraperitoneally injected. In celecoxib group, the liver cancer model was also established, and celecoxib was intraperitoneally injected. After intervention for 30 d, the samples were taken. The body weight of rats was measured before modeling and before sampling. The morphology of liver tissues was observed via hematoxylin-eosin (HE) staining, the expressions of related proteins and messenger ribonucleic acids (mRNAs) were determined via Western blotting and quantitative polymerase chain reaction (qPCR), respectively, and the protein expressions of cysteinyl aspartate specific proteinase 3 (Caspase3) and Cyclin D1 in liver tissues were detected.
Before modeling, there was no difference in t.
通过细胞外信号调节激酶（ERK）/c-Jun N-末端激酶（JNK）/p38 通路研究塞来昔布对肝癌大鼠的作用。
将 36 只 Sprague-Dawley 大鼠随机分为 3 组（每组 12 只）。在模型组中，建立肝癌模型，腹腔内注射生理盐水。在塞来昔布组中，也建立肝癌模型，腹腔内注射塞来昔布。干预 30 d 后取样本。在建模前和取样前测量大鼠体重。通过苏木精-伊红（HE）染色观察肝组织形态，通过 Western blot 和实时聚合酶链反应（qPCR）分别测定相关蛋白和信使核糖核酸（mRNA）的表达，检测肝组织中半胱氨酸天冬氨酸特异性蛋白酶 3（Caspase3）和细胞周期蛋白 D1 的蛋白表达。
在建模前，t 无差异。
