芸薹属植物游离小孢子培养议定书。
Brassica rapa L. ssp. chinensis Isolated Microspore Culture Protocol.
机构信息
Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center, VNIISSOK, Russian Federation.
出版信息
Methods Mol Biol. 2021;2288:145-162. doi: 10.1007/978-1-0716-1335-1_9.
PMID:34270010
Abstract
Culture of isolated microspores is a widely used method to obtain haploid and doubled haploid plants for many crop species. This protocol describes the steps necessary to obtain a large number of microspore derived embryos for pakchoi (Brassica rapa L. ssp. chinensis) and zicaitai (Brassica rapa L. ssp. сhinensis Hanelt var. purpuraria Kitam).
摘要
孤雌生殖培养是一种广泛应用于许多作物的方法，可获得单倍体和双单倍体植株。本方案描述了获得大量小白菜（芸薹属白菜亚种）和紫菜薹（芸薹属白菜亚种汉菜变种）小孢子胚的必要步骤。
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