Gupta G, Sarma M H, Sarma R H, Bald R, Engelke U, Oei S L, Gessner R, Erdmann V A
Institute of Biomolecular Stereodynamics, State University of New York at Albany 12222.
Biochemistry. 1987 Dec 1;26(24):7715-23. doi: 10.1021/bi00398a027.
A hairpin structure contains two conformationally distinct domains: a double-helical stem with Watson-Crick base pairs and a single-stranded loop that connects the two arms of the stem. By extensive 1D and 2D 500-MHz 1H NMR studies in H2O and D2O, it has been demonstrated that the DNA oligomers d(CGCCGCAGC) and d(CGCCGTAGC) form hairpin structures under conditions of low concentration, 0.5 mM in DNA strand, and low salt (20 mM NaCl, pH 7). From examination of the nuclear Overhauser effect (NOE) between base protons H8/H6 and sugar protons H1' and H2'/H2", it was concluded that in d(CGCCGCAGC) and d(CGCCGTAGC) all the nine nucleotides display average (C2'-endo,anti) geometry. The NMR data in conjunction with molecular model building and solvent accessibility studies were used to derive a working model for the hairpins.
一个具有沃森-克里克碱基对的双螺旋茎和一个连接茎的两条臂的单链环。通过在H2O和D2O中进行广泛的一维和二维500兆赫兹1H NMR研究，已证明DNA寡聚物d（CGCCGCAGC）和d（CGCCGTAGC）在低浓度（DNA链浓度为0.5 mM）和低盐（20 mM NaCl，pH 7）条件下形成发夹结构。通过检查碱基质子H8/H6与糖质子H1'和H2'/H2"之间的核Overhauser效应（NOE），得出结论：在d（CGCCGCAGC）和d（CGCCGTAGC）中，所有九个核苷酸均呈现平均（C2'-内，反式）几何形状。结合分子模型构建和溶剂可及性研究的NMR数据被用于推导发夹的工作模型。
