通过皮秒吸收光谱法研究α和β血红蛋白链中CO和O₂的光解离。
Photodissociation of CO and O2 from alpha and beta hemoglobin chains studied by using picosecond absorption spectroscopy.
作者信息
Guest C R, Noe L J
机构信息
Department of Chemistry, University of Wyoming, Laramie 82071.
出版信息
Biophys J. 1987 Nov;52(5):885-9. doi: 10.1016/S0006-3495(87)83282-4.
Abstract
The picosecond photodissociation of the CO and O2 forms of alpha and beta chains of hemoglobin were studied by following pi pi Soret absorption changes using a Nd3+ phosphate-glass laser, 531-nm pump pulse, 8 ps full width half maximum, and a pump-probe double-beam absorption apparatus. Three intermediates were observed within the first 50 ps after photon absorption. The most notable differences between the two monomers are the extent and rate of geminate recombination with the two ligands. We attribute this result to differences between the tertiary protein structure of the alpha and beta forms of Hb, both distal and proximal.
摘要
利用钕掺杂磷酸盐玻璃激光器、531纳米泵浦脉冲、半高宽为8皮秒的泵浦脉冲以及泵浦-探测双光束吸收装置，通过跟踪π-π索雷特吸收变化，研究了血红蛋白α链和β链的CO和O₂形式的皮秒光解离。在光子吸收后的前50皮秒内观察到了三种中间体。两种单体之间最显著的差异在于与两种配体的双分子复合程度和速率。我们将此结果归因于Hb的α型和β型三级蛋白质结构在远侧和近侧的差异。
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