Zhejiang Provincial Key Laboratory of Petrochemical Pollution Control, School of Petrochemical Engineering & Environment, Zhejiang Ocean University, Zhoushan, 316022, China; College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, 316022, China.
College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, 316022, China.
Chemosphere. 2021 Dec;285:131475. doi: 10.1016/j.chemosphere.2021.131475. Epub 2021 Jul 12.
This study was aimed to assess the growth and lipid productivity improvement of a green microalga Scenedesmus obliquus by mixotrophic cultivation, via addition of sodium acetate (NaAc) into actual municipal wastewater (AMW). Moreover, the nutrient removal efficiency of the culture media in terms of carbon, nitrogen, and phosphorus was investigated. The results showed that the S. obliquus grew better in the AMW than in the BG11 medium (0.20 g L vs 0.16 g L in dry cell weight), and the final algal lipid productivity was higher (9.02 mg L d vs 7.75 mg L d, P < 0.05). Further, the addition of NaAc significantly stimulated the algal growth and lipid productivity. Specifically, the highest improvement was obtained by the NaAc-addition of 1 g L, where the algal dry cell weight increased 2.40 times than that in the AMW with little organic carbon (0.48 mg L vs 0.20 mg L, P < 0.01), and the corresponding algal lipid productivity increased 2.44 time (22.08 mg L d vs 9.02 mg L d, P < 0.01). Meanwhile, the addition of 1 g L of NaAc significantly increased the microalga-driven nitrogen and phosphorus removal efficiency, respectively by 1.75 and 2.23 times (82.20% vs 46.85% for total nitrogen, and 76.35% vs 34.18% for total phosphorus). In summary, this study confirmed the feasibility of using organic carbon-supplemented AMW to replace the artificial media for microalgae-based lipid production and nutrient recycling.
本研究旨在通过向实际城市污水（AMW）中添加乙酸钠（NaAc），评估混养培养对斜生栅藻（Scenedesmus obliquus）生长和油脂生产力提高的作用。此外，还研究了培养基在碳、氮和磷方面的营养去除效率。结果表明，与 BG11 培养基相比（干重分别为 0.20 g/L 和 0.16 g/L），斜生栅藻在 AMW 中生长得更好，最终的藻类油脂生产力更高（9.02 mg/L/d 和 7.75 mg/L/d，P<0.05）。此外，添加 NaAc 显著刺激了藻类的生长和油脂生产力。具体而言，添加 1 g/L NaAc 可获得最大的改善，此时藻类干重比 AMW 中的有机碳（0.48 mg/L 和 0.20 mg/L，P<0.01）增加了 2.40 倍，相应的藻类油脂生产力增加了 2.44 倍（22.08 mg/L/d 和 9.02 mg/L/d，P<0.01）。同时，添加 1 g/L NaAc 分别显著提高了微藻驱动的氮和磷去除效率，分别提高了 1.75 倍和 2.23 倍（总氮分别为 82.20%和 46.85%，总磷分别为 76.35%和 34.18%）。综上所述，本研究证实了使用含碳有机补充的 AMW 替代人工培养基用于微藻油脂生产和营养回收的可行性。
