Ferssiwi A, Cardo B, Velley L
Laboratoire de Psychophysiologie, Talence, France.
Brain Res. 1987 Dec 22;437(1):142-50. doi: 10.1016/0006-8993(87)91535-6.
The main purpose of this study was to quantitate possible changes in the rewarding and aversive values of certain gustatory stimuli produced by bilateral ibotenic acid lesions of the lateral hypothalamus in the rat. Non-operated rats served as controls. Thirteen days after the operation, rats were placed on a water-deprivation schedule during 5 consecutive days. Rats were then given the choice of one of 5 concentrations of saccharin solution, using a two-bottle procedure. Fluid intake across concentrations generated a preference-aversion curve. The same type of procedure was used to obtain the aversion curve for increasing concentrations of quinine solution. The lesioned rats as well as the control animals showed a clear preference-aversion response to saccharin solutions and an aversive response to quinine solutions. However, the highest preference score of the lesioned rats was obtained with a saccharin concentration 3 times higher than the concentration preferred by the control rats. Moreover, unlike control rats operated animals did not show aversion to the highest concentrations of saccharin solutions. Finally in the lesioned rats the aversion threshold to quinine solutions was obtained with concentration 5 times higher than the concentration inducing aversion in the control rats. At the end of these experiments, rats used as controls were submitted, in turn, to bilateral lesion of the lateral hypothalamus. The change in the preference-aversion threshold of these rats in the saccharin choice procedure was the same as that observed with naive rats. Taken together, these results suggest that in the normal rat the palatability of certain gustatory stimuli is modulated by the intrinsic neurons of the lateral hypothalamus.
本研究的主要目的是定量分析大鼠双侧下丘脑外侧注射鹅膏蕈氨酸损伤后某些味觉刺激的奖赏和厌恶值可能发生的变化。未做手术的大鼠作为对照。手术后13天，大鼠连续5天接受限水安排。然后采用双瓶法让大鼠在5种不同浓度的糖精溶液中进行选择。不同浓度下的液体摄入量生成了一条偏好-厌恶曲线。采用相同的方法获取不同浓度奎宁溶液的厌恶曲线。损伤组大鼠和对照动物对糖精溶液均表现出明显的偏好-厌恶反应，对奎宁溶液表现出厌恶反应。然而，损伤组大鼠的最高偏好分数对应的糖精浓度比对照组大鼠偏好的浓度高3倍。此外，与对照大鼠不同，手术动物对最高浓度的糖精溶液未表现出厌恶。最后，损伤组大鼠对奎宁溶液的厌恶阈值对应的浓度比诱导对照大鼠产生厌恶的浓度高5倍。在这些实验结束时，依次对用作对照的大鼠进行双侧下丘脑外侧损伤。这些大鼠在糖精选择实验中偏好-厌恶阈值的变化与未做处理的大鼠所观察到的相同。综上所述，这些结果表明，在正常大鼠中，某些味觉刺激的适口性受下丘脑外侧固有神经元的调节。
