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人骨髓间充质干细胞与血小板共移植鼠创面的转录组学分析。

Transcriptional analysis of mouse wounds grafted with human mesenchymal stem cells and platelets.

机构信息

Univ Paris Est Creteil, INSERM, IMRB, 94010 Créteil, France.

出版信息

STAR Protoc. 2021 Jul 3;2(3):100650. doi: 10.1016/j.xpro.2021.100650. eCollection 2021 Sep 17.

DOI:10.1016/j.xpro.2021.100650
PMID:34278336
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8261014/
Abstract

Platelet preparations are commonly used in the clinic in combination with mesenchymal stem cells (MSCs) to improve their wound healing capacity and optimize their therapeutic efficacy following their delivery into diseased tissues. To investigate the mechanisms by which platelets enhance the repair properties of MSCs, we detail a protocol using a humanized mouse model for excisional wounds to study by reverse transcription real-time PCR whether human platelets alter the therapeutic efficacy of grafted human MSCs. For complete details on the use and execution of this protocol, please refer to Levoux et al. (2021).

摘要

血小板制剂通常与间充质干细胞 (MSCs) 联合应用于临床，以提高其在进入病变组织后的伤口愈合能力并优化其治疗效果。为了研究血小板增强 MSCs 修复特性的机制，我们详细介绍了一种使用人源化小鼠模型进行切创的方案，通过逆转录实时 PCR 研究人血小板是否改变了移植的人 MSCs 的治疗效果。如需了解本方案的使用和实施的详细信息，请参阅 Levoux 等人 (2021)。

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本文引用的文献

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Cell Metab. 2021 Feb 2;33(2):283-299.e9. doi: 10.1016/j.cmet.2020.12.006. Epub 2021 Jan 4.
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Evaluation of the stability of standard reference genes of adipose-derived mesenchymal stem cells during in vitro proliferation and differentiation.评估脂肪间充质干细胞在体外增殖和分化过程中标准参考基因的稳定性。
Mol Biol Rep. 2020 Mar;47(3):2109-2122. doi: 10.1007/s11033-020-05311-y. Epub 2020 Feb 17.
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Assessing clinical implications and perspectives of the pathophysiological effects of erythrocytes and plasma free hemoglobin in autologous biologics for use in musculoskeletal regenerative medicine therapies. A review.
评估红细胞和血浆游离血红蛋白在用于肌肉骨骼再生医学治疗的自体生物制品中的病理生理效应的临床意义和前景。一篇综述。
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Effects of Different Cell-Detaching Methods on the Viability and Cell Surface Antigen Expression of Synovial Mesenchymal Stem Cells.不同细胞分离方法对滑膜间充质干细胞活力及细胞表面抗原表达的影响
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Primer3--new capabilities and interfaces.Primer3--新功能和界面。
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Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse.来自人类脂肪组织的多能细胞群移植可诱导免疫活性mdx小鼠表达抗肌萎缩蛋白。
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