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从小鼠骨骼肌和心肌中分离出的肌膜和肌浆网组分的比较生化研究。

Comparative biochemical study of sarcolemma and sarcoplasmic reticulum fractions isolated from mouse skeletal and cardiac muscles.

作者信息

Lucas-Heron B, Loirat M J, Ollivier B

机构信息

Laboratoire de Physiologie, U.E.R. de Médecine, Nantes, France.

出版信息

Comp Biochem Physiol B. 1987;88(2):421-7. doi: 10.1016/0305-0491(87)90322-1.

Abstract
  1. Ca-ATPase activity, calcium-binding proteins and Concanavalin-A-bound glycoproteins of sarcolemma and sarcoplasmic reticulum were compared in mouse cardiac and skeletal muscles. 2. Ca-ATPase activity and calsequestrin were quite reduced in cardiac muscle, and the quantity of calcium bound to these two proteins was practically negligible, contrary to what was observed with skeletal muscle. In addition, the quantity of lipid bound calcium was not greater in cardiac muscle than in skeletal muscle. 3. Certain proteins seemed exclusively specific for skeletal muscle, including a 30,000 mol. wt glycoprotein which was totally absent in cardiac muscle sarcolemma.
摘要
  1. 比较了小鼠心肌和骨骼肌中肌膜和肌浆网的钙 - ATP酶活性、钙结合蛋白以及伴刀豆球蛋白A结合糖蛋白。2. 心肌中的钙 - ATP酶活性和肌集钙蛋白显著降低,与骨骼肌中观察到的情况相反,结合到这两种蛋白上的钙量实际上可以忽略不计。此外,心肌中结合脂质的钙量并不比骨骼肌中的多。3. 某些蛋白质似乎是骨骼肌特有的,包括一种分子量为30,000的糖蛋白,它在心肌肌膜中完全不存在。

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