Morphology and ultrastructure of the epithelial femoral gland in cicadas (Hemiptera: Cicadidae).

Exocrine glands in the legs of social insects are found throughout all leg segments, but studies of exocrine glands in legs of solitary insects are very limited. We discovered a novel gland at the apex of the fore, mid and hind femurs from six representative species of Cicadidae, which we propose to name as the epithelial femoral gland. The epithelial femoral gland is located between the paired apodemes and the articulation membrane within the apex of the femur, which faces the proximal articulation region of the tibia. The epithelial femoral gland in the midlegs is less developed than that in the fore- and hindlegs within a species. The glandular cells belong to class-1, which contain a large amount of rough endoplasmic reticulum, secretory vesicles and Golgi bodies, indicating that these cells may produce a proteinaceous secretion. Details of the epithelial femoral gland at the ultrastructural level suggest that it may function to produce nourishing substances to the joint between femur and tibia. The less developed epithelial femoral gland in the midlegs and the slight difference in the glands between fore- and hindlegs within a species could be related to the functional differentiation of the corresponding legs in cicadas. Further studies of exocrine glands in the legs of cicadas and other Cicadomorpha insects may improve our understanding of the structural and functional divergence of legs in hemipteran insects.