Department of Laboratory Medicine, Chair of Microbiology, Immunology and Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland.
Department of Diagnostic Immunology, Chair of Microbiology, Immunology and Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland.
Int J Mol Sci. 2021 Jul 1;22(13):7104. doi: 10.3390/ijms22137104.
This study evaluates the electrical potential and chemical alterations in laboratory-induced colistin-resistant , as compared to the susceptible strain using spectroscopic analyses. The minimal inhibitory concentration (MIC) of colistin, ζ-potential and chemical composition analysis of strains are determined. The results obtained for the with induced high-level colistin resistance (MIC = 16.0 ± 0.0 mg/L) are compared with the strain susceptible to colistin (MIC = 0.25 ± 0.0 mg/L). Fourier transform infrared (FTIR) and Raman spectroscopic studies revealed differences in bacterial cell wall structures and lipopolysaccharide (LPS) of and strains. In the beginning, we assumed that the obtained results could relate to a negative charge of the bacterial surface and different electrostatic interactions with cationic antibiotic molecules, reducing the affinity of colistin and leading to its lower penetration into cell. However, no significant differences in the ζ-potential between the and strains are noticed. In conclusion, this mechanism is most probably associated with recognisable changes in the chemical composition of the cell wall (especially in LPS) when compared to the susceptible strain.
本研究使用光谱分析评估了实验室诱导的粘菌素耐药株与敏感株之间的电化学变化。测定了粘菌素的最小抑菌浓度（MIC）、ζ-电位和菌株的化学成分分析。将诱导产生高水平粘菌素耐药性的（MIC=16.0±0.0mg/L）与对粘菌素敏感的（MIC=0.25±0.0mg/L）进行比较。傅里叶变换红外（FTIR）和拉曼光谱研究揭示了 株和 株细菌细胞壁结构和脂多糖（LPS）的差异。起初，我们假设获得的结果可能与细菌表面的负电荷和与阳离子抗生素分子的不同静电相互作用有关，从而降低了粘菌素的亲和力，导致其更难进入 细胞。然而，在 株和 株之间未观察到 ζ-电位的显著差异。总之，与敏感株相比，这种机制很可能与 细胞壁化学成分（特别是 LPS）的可识别变化有关。
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