傅里叶变换红外光谱法作为分析奇异变形杆菌内毒素的一种工具
Fourier Transform Infrared Spectroscopy as a Tool in Analysis of Proteus mirabilis Endotoxins.
作者信息
Żarnowiec Paulina, Czerwonka Grzegorz, Kaca Wiesław
机构信息
Department of Microbiology, Jan Kochanowski University, Świętokrzyska 15, PL-25-406, Kielce, Poland.
出版信息
Methods Mol Biol. 2017;1600:113-124. doi: 10.1007/978-1-4939-6958-6_11.
Fourier transform infrared spectroscopy (FT-IR) was used to scan whole bacterial cells as well as lipopolysaccharides (LPSs, endotoxins) isolated from them. Proteus mirabilis cells, with chemically defined LPSs, served as a model for the ATR FT-IR method. The paper focuses on three steps of infrared spectroscopy: (1) sample preparation, (2) IR scanning, and (3) multivariate analysis of IR data (principal component analysis, PCA).
傅里叶变换红外光谱法（FT-IR）用于扫描整个细菌细胞以及从这些细胞中分离出的脂多糖（LPS，内毒素）。具有化学定义的LPS的奇异变形杆菌细胞用作衰减全反射傅里叶变换红外光谱（ATR FT-IR）方法的模型。本文重点介绍红外光谱的三个步骤：（1）样品制备，（2）红外扫描，以及（3）红外数据的多变量分析（主成分分析，PCA）。
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