School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
J Equine Vet Sci. 2021 Aug;103:103669. doi: 10.1016/j.jevs.2021.103669. Epub 2021 Jun 6.
Uterocalin (P19) is a lipocalin protein that has binding activity for the provisioning of the embryo with lipids, including those essential for morphogenesis and pattern formation. Therefore, it is thought that P19 plays an important role in supporting the survival of the early embryo. Previous research has shown that the endometrium from acyclic mares expressed more P19 following a simulated long estrus: treatment of 8 mg of estradiol benzoate (EB) split over five consecutive days, followed by long-acting progesterone administration (LA-P4) 72 hours later, when compared to a single 2.5 mg EB treatment. The main objective of this study was to evaluate if a new long estrus protocol, with fewer EB administrations, also increases endometrial uterocalin expression. Fifteen mares were assigned to three groups: long estrus 5 administrations (LE5; a total of 8 mg of EB in increasing doses was given over five consecutive days, Day -7 to Day -3); long estrus 2 administrations (LE2; 3 and 5 mg EB were given on Day -7 and Day -4); and short estrus (SE; a single dose of 2.5 mg EB was administered on Day -2). All groups received LA P4 on day 0 (D0). Daily ultrasonography and blood collections were performed to assess uterine edema and estradiol and progesterone plasma concentrations, respectively. Endometrial biopsies were collected on Day 4 to evaluate P19 expression by RT-qPCR. There were no differences in P19 expression between groups LE2 and LE5. However, P19 expression was higher (P < .05) in the LE groups than in the SE group. Both LE protocols produced high uterine edema (score 2-3) for at least 6 days. There was no effect of group (LE2 or LE5) on overall estradiol and progesterone concentrations (P > .1). In conclusion, the two EB administrations protocol is more feasible for preparing acyclic recipient mares at field conditions. However, a positive effect of the LE protocols on embryo survival and viability in anestrous treated mares still needs to be confirmed.
尿钙结合蛋白（P19）是一种脂联素蛋白，具有为胚胎提供脂质的结合活性，包括对形态发生和模式形成至关重要的脂质。因此，人们认为 P19 在支持早期胚胎的存活方面发挥着重要作用。先前的研究表明，在模拟长发情期间，非发情母马的子宫内膜表达更多的 P19：连续五天每天给予 8 毫克苯甲酸雌二醇（EB），然后在 72 小时后给予长效孕激素（LA-P4），与单次 2.5 毫克 EB 处理相比。本研究的主要目的是评估新的长发情方案，即减少 EB 给药次数，是否也会增加子宫内膜尿钙结合蛋白的表达。将 15 匹母马分为三组：长发情 5 次（LE5；总剂量为 8 毫克的 EB，连续 5 天，在 Day-7 至 Day-3 时逐渐增加）；长发情 2 次（LE2；在 Day-7 和 Day-4 时给予 3 和 5 mg EB）；和短发情（SE；在 Day-2 时给予单剂量 2.5 mg EB）。所有组均在第 0 天（D0）给予 LA-P4。每天进行超声检查和采血，以分别评估子宫水肿和雌二醇和孕酮的血浆浓度。在第 4 天收集子宫内膜活检，通过 RT-qPCR 评估 P19 的表达。在 LE2 和 LE5 组之间，P19 的表达没有差异。然而，LE 组的 P19 表达高于 SE 组（P <.05）。两种 LE 方案都至少在 6 天内产生了高子宫水肿（评分 2-3）。LE 方案（LE2 或 LE5）对雌二醇和孕酮的总浓度没有影响（P >.1）。总之，两种 EB 给药方案在现场条件下更适合准备非发情受体母马。然而，LE 方案对发情处理的乏情母马胚胎存活和活力的积极影响仍需确认。
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